食品与机械2025，Vol.41Issue(5)：27-35,9.DOI:10.13652/j.spjx.1003.5788.2024.80912
Fe3O4-SA/CS微球固定菠萝蛋白酶及其酶学性质研究
Immobilization of bromelain on Fe3O4-SA/CS microspheres and its enzymatic properties
摘要
Abstract
[Objective]To improve the immobilization technology of bromelain which has poor stability to enhance its utilization,stability,and storage performance.[Methods]The preparation process of magnetic chitosan/sodium alginate immobilized bromelain(Fe3O4-SA/CS-Br)is optimized by the response surface methodology.The Fe3O4-SA/CS-Br is characterized and its Km value,operational stability,thermal stability,storage stability,and application effectiveness are determined.[Results]The optimal preparation conditions for Fe3O4-SA/CS-Br are as follows:mchitosan∶msodium alginate ratio of 1∶4,Fe3O4 mass concentration of 10 mg/mL,CaCl2 mass concentration of 32 mg/mL,immobilization time of 106 min,pH value of 7.0,and enzyme mass concentration of 1.0 mg/mL.According to the results from scanning electron microscopy,infrared spectrum,and X-ray diffraction spectrum,the main functional groups of bromelain are successfully immobilized on Fe3O4-chitosan/sodium alginate(Fe3O4-SA/CS)microspheres.The affinity of bromelain for the substrate is enhanced after immobilization.[Conclusion]Fe3O4-SA/CS-Br can effectively enhance the stability of bromelain.关键词
磁性微球/壳聚糖/海藻酸钠/固定化/菠萝蛋白酶/酶学性质Key words
magnetic microsphere/chitosan/sodium alginate/immobilization/bromelain/enzymatic property引用本文复制引用
王丹,耿丽晶,黄薇,付航,刘晓宇,Muhammad Zain Ul Aabideen,周围..Fe3O4-SA/CS微球固定菠萝蛋白酶及其酶学性质研究[J].食品与机械,2025,41(5):27-35,9.基金项目
辽宁省应用基础研究计划(编号:2023JH2/101300172) （编号:2023JH2/101300172）
锦州医科大学大学生创新创业项目(编号:X202410160009X) （编号:X202410160009X）
辽宁省省级大学生创新创业项目(编号:S202310160003X) （编号:S202310160003X）
