食品与机械2025，Vol.41Issue(5)：64-70,7.DOI:10.13652/j.spjx.1003.5788.2024.80913
离子色谱法测定婴幼儿配方乳粉中6种母乳寡聚糖
Determination of six human milk oligosaccharides in infant formula milk powder by ion chromatography
摘要
Abstract
[Objective]To establish an ion chromatography method for the simultaneous determination of six human milk oligosaccharides(HMOs)in infant formula powder.[Methods]The samples were enzymatically hydrolyzed using fructanase.Gradient elution was performed with a mobile phase consisting of water,0.5 mol/L sodium hydroxide,and 0.3 mol/L sodium acetate.Separation was carried out on a Thermo Fisher CarboPac™ PA1 Analytical Column,and detection was performed using ion chromatography with pulsed amperometric detection.[Results]In milk powder,2'-fucosyllactose(2'-FL),3-fucosyllactose(3-FL),and lacto-N-tetraose(LNT)showed good linearity within the range of 1～100 mg/L,while lacto-N-neotetraose(LNnT),3'-sialyllactose(3'-SL),and 6'-sialyllactose(6'-SL)exhibited good linearity in the range of 0.5～50.0 mg/L,with correlation coefficients all greater than 0.999.The limit of detection(LOD)for 2'-FL,LNnT,3-FL,and LNT was 10 mg/100 g,and the limit of quantification(LOQ)was 30 mg/100 g.The LOD for 3'-SL and 6'-SL was 5 mg/100 g,and the LOQ was 15 mg/100 g.The average recoveries of the six HMOs at low,medium,and high concentrations ranged from 98.0％ to 101.0％,with relative standard deviations(RSDs)between 0.3％ and 2.4％.In actual sample analysis,when the content of the six HMOs ranged from 0.1 to 10.0 g/kg,the RSDs were 0.5％ to 9.4％.When the content ranged from 10 to 100 g/kg,the RSDs were 1.4％ to 4.0％.[Conclusion]The proposed method meets the requirements for routine analysis and is suitable for the determination of six HMOs in various infant formula powders containing HMOs.关键词
离子色谱法/酶解法/母乳寡聚糖/婴幼儿配方乳粉Key words
ion chromatography/enzymolysis/human milk oligosaccharides/infant formula milk powder引用本文复制引用
张嘉杞,李云萱,王璐璐,张水锋,潘项捷,施元旭,阙利明..离子色谱法测定婴幼儿配方乳粉中6种母乳寡聚糖[J].食品与机械,2025,41(5):64-70,7.基金项目
浙江省市场监督管理局科技计划(编号:ZC2023046) （编号:ZC2023046）
