食品与发酵工业2025，Vol.51Issue(12)：36-43,8.DOI:10.13995/j.cnki.11-1802/ts.040348
木姜子提取物对大肠杆菌的抑菌机理及其在鱼肉保鲜中的应用
Antibacterial mechanism of Litsea cubeba extract against Escherichia coli and its application in fish preservation
摘要
Abstract
This study aimed to elucidate the antibacterial mechanism of Litsea cubeba ethanol extract(LCEE)and explore its poten-tial application in food preservation.This investigation involved a comprehensive analysis of the antibacterial activity of LCEE,focusing on its effects on cell wall-membrane damage,decrease in energy substances,and morphological changes observed through electron microsco-py,using Escherichia coli as the model organism.Additionally,the effectiveness of LCEE in fish preservation was assessed through experi-mental trials.Results revealed that LCEE possessed significant antibacterial activity against a variety of foodborne pathogens.LCEE caused continuous leakage of intracellular ions,proteins,nucleic acids,and alkaline phosphatase by damaging the bacterial cell walls and mem-branes,and caused a significant decrease in intracellular ATP levels.The surface of cell treated with LCEE showed wrinkles collapses and holes,and the leakage of cell contents led to the emergence of a large number of intracellular hollow cavities.Furthermore,the use of LCEE has been shown to enhance the water holding capacity of fish during storage,delay discoloration,and control the increase of total volatile basic nitrogen levels,total colony counts,and coliform counts,as well as extend the shelf life of fish for more than 2 days.In con-clusion,the discovery of LCEE has further enriched the antimicrobial constituents of Litsea cubeba fruits,which inhibit the growth of Esch-erichia coli through multiple mechanisms,with promising results in food preservation.关键词
木姜子提取物/大肠杆菌/抗菌机制/壁膜损伤/鱼肉保鲜Key words
Litsea cubeba ethanol extract/Escherichia coli/antibacterial mechanism/cell wall-membrane damage/fish preservation引用本文复制引用
刘梦龙,陈聪,陆开祥,魏美娟,胡永金,薛桥丽..木姜子提取物对大肠杆菌的抑菌机理及其在鱼肉保鲜中的应用[J].食品与发酵工业,2025,51(12):36-43,8.基金项目
国家自然科学基金地区项目(32060520) （32060520）
云南省科技人才与平台计划项目(202105AF150049) （202105AF150049）
