食品与发酵工业2025，Vol.51Issue(12)：84-91,8.DOI:10.13995/j.cnki.11-1802/ts.040201
可代谢半乳糖嗜热链球菌IMAU20153Y的诱变选育及其特性分析
Mutagenic selection of metabolizable galactose Streptococcus thermophilus IMAU20153 Y and its characterization
摘要
Abstract
Streptococcus thermophilus is an important starter culture widely used in dairy fermentation.Most S.thermophilus strains o-riginating from milk can not use the galactose moiety of lactose,resulting in high galactose content in fermented milk.In this study,S.thermophilus IMAU20153 which was known to have good fermentation characteristics,was treated by using the chemical mutagen agent N-methyl-N′-nitro-N-nitrosoguanidine(NTG),which can be metabolize galactose,and used for the industrial production of fermented milk with low levels of galactose.S.thermophilus IMAU20153Y,which can metabolize galactose,was screened by comparing the enzyme activi-ty assays,fermentation time and sequencing.HPLC analysis demonstrated that S.thermophilus IMAU20153Y enhanced ability to utilize lactose and galactose,leading to increased lactic acid.These findings offer new insights on the development and application of fermented milk with low levels of galactose.关键词
嗜热链球菌/亚硝基胍/诱变/半乳糖Key words
Streptococcus thermophilus/N-methyl-N′-nitro-N-nitrosoguanidine/mutant/galactose引用本文复制引用
卢纹慧,邰佳慧,吴娜,刘金惠,丹彤..可代谢半乳糖嗜热链球菌IMAU20153Y的诱变选育及其特性分析[J].食品与发酵工业,2025,51(12):84-91,8.基金项目
国家自然科学基金项目(32072235) （32072235）
内蒙古自然科学基金面上项目(2022MS03013) （2022MS03013）
