食品与发酵工业2025，Vol.51Issue(12)：92-99,8.DOI:10.13995/j.cnki.11-1802/ts.039486
pH响应性金属-多酚网络包覆纳米颗粒的抗炎活性研究
Anti-inflammatory activity study of pH-responsive metal-polyphenol structure-coated nanoparticles
摘要
Abstract
To investigate the anti-inflammatory activity of pH-responsive polyphenol delivery systems,zein-phlorotannin(PTN)-car-bon quantum dots(CQDs)nanoparticles were constructed by loading PTN into zein-CQDs nanoparticles.Zein-PTN-CQDs-FeⅢ nanoparti-cles were further prepared by adding tannic acid and Fe3+based on zein-PTN-CQDs nanoparticles.The pH-responsive release characteris-tics and anti-inflammatory activity of the nanoparticles were explored through experiments on polyphenol release in vitro,cell toxicity,cell apoptosis imaging,anti-inflammatory properties,and reactive oxygen species(ROS)detection.Results showed that the addition of CQDs effectively reduced the particle size and increased the encapsulation efficiency.pH-responsive polyphenol-metal coating networks were formed on the surface of zein-PTN-CQDs nanoparticles through the combination of tannic acid and Fe3+.The release rates of PTN-CQDs-FeIII nanoparticles under acidic conditions of pH 6.2,5,and 4 were 78%,89%,and 96%,respectively,significantly higher than those under neutral conditions(28%),exhibiting sustained release and pH-responsive characteristics.For lipopolysaccharide-stimulated RAW 264.7 cells,zein-PTN-CQDs-FeⅢ nanoparticles significantly reduced ROS production and exhibited superior inhibition of pro-inflammatory factors(IL-1β,IL-6,and TNF-α)and promotion of anti-inflammatory factors(IL-10 and prostaglandin E2)compared to the PTN group and zein-CQDs-FeⅢ group.关键词
金属/褐藻多酚/纳米颗粒/pH响应/抗炎活性Key words
metal/phlorotannins/nanoparticles/pH-responsive/anti-inflammatory activity引用本文复制引用
干淼钰,臧运芳,曾凡正,曹爱玲,梁艳,刘全胜,蔡路昀,李钰金..pH响应性金属-多酚网络包覆纳米颗粒的抗炎活性研究[J].食品与发酵工业,2025,51(12):92-99,8.基金项目
宁波市公益重点项目(2022S148) （2022S148）
宁波市重点研发计划项目(2024Z282,2024Z277) （2024Z282,2024Z277）
国家重点研发计划项目(2018YFD0901106) （2018YFD0901106）
温州市重大科技专项(ZN2021002) （ZN2021002）
宁波市高层次人才"3315系列计划"项目(2020B-34-G) （2020B-34-G）
