食品与发酵工业2025，Vol.51Issue(12)：107-114,8.DOI:10.13995/j.cnki.11-1802/ts.040334
腾冲姜黄精油的成分及其对变异链球菌抑菌机理的初探
Composition of Tengchong turmeric essential oil and preliminary study on its antibacterial mechanism against Streptococcus mutans
摘要
Abstract
The antibacterial activity of Tengchong turmeric essential oil against Streptococcus mutans was investigated to explore its main active components and potential mechanism of action.The major components of the essential oil were analyzed by gas chromatogra-phy-mass spectrometry combined with Kovats retention index.S.mutans was treated with different concentrations of essential oil.The chan-ges in its microscopic morphology,surface charge,membrane integrity,and electrical conductivity were observed before and after treat-ment.Results showed that the major components of turmeric essential oil were α-curcumene,α-zingiberene,β-bisabolene,β-sesquiphel-landrene,ar-turmerone,turmerone,and β-curcumin.The minimum inhibitory concentration(MIC)of essential oil against S.mutans was 0.25 mg/mL and the minimum bactericidal concentration(MBC)was0.5 mg/mL.After adding turmeric essential oil with the minimum inhibitory concentration to the culture medium,the growth of S.mutans was completely inhibited.The cell membranes of some bacteria were ruptured,intracellular substances flowed out,the surface charge decreased,and some S.mutans cells were damaged.The electrical conductivity showed a relatively higher increasing trend at 0-4 hours and increased more slowly at 4-8 hours.However,after adding tur-meric essential oi with the minimum bactericidal concentration to the culture medium,the growth of S.mutans had stagnated and even been killed.The cell clusters were adhered together,the stability of the bacteria became worse,all the cell membranes of the bacteria were de-stroyed,and the ions inside the cells were released to the external environment of the bacteria.The significant increase in electrical con-ductivity led to cell death.Compared with the control group,turmeric essential oil has the effect of inhibiting S.mutans.The research re-sults provide an important reference basis for the prevention and treatment of dental caries with turmeric essential oil.关键词
姜黄精油/变异链球菌/抑菌活性/抑菌机理Key words
turmeric essential oil/Streptococcus mutans/antibacterial activity/antibacterial mechanism引用本文复制引用
杨晓娜,徐玲,李田,张翼飞,陈自宏..腾冲姜黄精油的成分及其对变异链球菌抑菌机理的初探[J].食品与发酵工业,2025,51(12):107-114,8.基金项目
国家自然科学地区基金项目(32060638) （32060638）
云南省教育厅教师类项目(2023J1122) （2023J1122）
云南省中青年学术和技术带头人后备人才项目(202205AC160061) （202205AC160061）
云南省教育厅高黎贡山虫生真菌资源保护与绿色发展重点实验室建设项目 （）
保山学院科研项目(22BYZY02) （22BYZY02）
