食品与发酵工业2025，Vol.51Issue(12)：115-122,8.DOI:10.13995/j.cnki.11-1802/ts.039965
高产单宁酶黑曲霉菌株的常温常压等离子体诱变选育及发酵工艺研究
Atmospheric room temperature plasma mutagenesis breeding and fermentation optimization of Aspergillus niger with high yield of tannase
摘要
Abstract
Tannase is a biocatalyst with high performance for hydrolyzing tannin substances.It has been widely used in various indus-tries such as food,beverage,and medicine.As an important microorganism for producing tannase,Aspergillus niger needs to further in-crease enzyme production through the breeding of high-yield strain and the optimization of fermentation process to meet the growing demand for industrial applications.In this study,the optimal conditions for atmospheric room temperature plasma(ARTP)mutagenesis in original strain Aspergillus niger LH were 3×106 CFU/mL spore concentration,100 W output power,10 L/min flow rate of the carrier gas,2 mm distance,and 240 s exposure time.Plate colorimetric analysis revealed that 49 mutants displayed large color-changing circle.According to shake flask fermentation,mutant LH2301 possessed the highest enzyme activity and stable enzyme production.The tannase activity reached 2.292 U/mL,2.27 times higher than the original strain.Through orthogonal experiments,the optimal cultivation conditions were 5 g/L glucose,21 g/L yeast extract powder,4.0 initial pH,3.5%inoculation amount,and 20 g/L tannic acid.The enzyme activity of the mu-tant strain could be increased to6.105 U/mL.Finally,the tannase produced from the mutant strain was investigated.Its molecular weight was 90 kDa.When the temperature was at 40-50℃and pH 3.0-5.0,the enzyme exhibited well catalytic activity.The optimal tempera-ture for this enzyme was 50℃and the optimal pH was 4.0,metal ions Ca2+,K+,and Al3+played a role in promoting enzyme activity.Our research expands the enzyme library resources of tannase and provides technical support for industrial application.关键词
黑曲霉/单宁酶/常温常压等离子体诱变/发酵/酶学性质Key words
Aspergillus niger/tannase/atmospheric room temperature plasma mutagenesis/fermentation/enzymatic properties引用本文复制引用
马东旭,李忠辉,张子恒,王昕,龚大春,石小涛..高产单宁酶黑曲霉菌株的常温常压等离子体诱变选育及发酵工艺研究[J].食品与发酵工业,2025,51(12):115-122,8.基金项目
国家自然科学基金项目(21776162) （21776162）
湖北省科技重大攻关项目(2018ACA121) （2018ACA121）
