食品与发酵工业2025，Vol.51Issue(12)：123-131,9.DOI:10.13995/j.cnki.11-1802/ts.040833
驼乳影响小鼠抗炎与免疫功能的代谢组学分析
Metabolomic analysis of effects of camel milk on anti-inflammatory and immune functions in mice
摘要
Abstract
This study investigated the effects of camel milk on the anti-inflammatory and immune functions of mice through plasma metabolomics and enzyme-linked immunosorbent assay(ELISA)analysis.A total of 32 ICR mice were randomly divided into two groups,including a camel milk group and a control group.The mice were orally administered 10 mL/kg body weight of camel milk or distilled wa-ter daily for 28 days.After the administration period,liver and blood samples were collected.Inflammatory markers in the mice were meas-ured using ELISA,and non-targeted metabolomics of serum metabolites was conducted using liquid chromatography-tandem mass spec-trometry(LC-MS/MS),followed by analysis of the results.Results demonstrated that camel milk administration elevated the levels of in-terleukin 6(IL-6),secretory immunoglobulin A(sIgA),interleukin 1β(IL-1β),and interferon γ(IFN-γ),while reducing tumor nec-rosis factor-α(TNF-α)levels in the liver of mice.A total of 207 differential metabolites were identified between the two groups,with 201 metabolites significantly upregulated and 6 significantly downregulated in the control group.Among these differential metabolites,lipid me-tabolites such as lysophosphatidylcholine(LPC)accounted for the majority,and these metabolites were significantly enriched in pathways including pyrimidine metabolism and the tricarboxylic acid(TCA)cycle.These findings suggest that camel milk may exert its anti-inflam-matory effects in mice by modulating lipid metabolism,pyrimidine metabolism,and the TCA cycle.The results provide valuable data for the functional development and utilization of camel milk.关键词
驼乳/代谢组学/抗炎作用/小鼠Key words
camel milk/metabolomics/anti-inflammatory/mice引用本文复制引用
刘师博,姚新奎,孟军,曾亚琦,孙志文,王建文..驼乳影响小鼠抗炎与免疫功能的代谢组学分析[J].食品与发酵工业,2025,51(12):123-131,9.基金项目
中央引导地方科技发展专项资金项目(ZYYD2023C02-2) （ZYYD2023C02-2）
自治区创新环境(人才、基地)建设专项(PT2311) （人才、基地）
自治区重大科技专项(2022A02013-1) （2022A02013-1）
