食品与发酵工业2025，Vol.51Issue(12)：183-190,8.DOI:10.13995/j.cnki.11-1802/ts.040575
富硒大豆蛋白的提取传质动力学及其结构性质研究
Extraction mass transfer kinetics and structural properties of selenium-enriched soybean protein
摘要
Abstract
The extraction process of selenium-enriched soybean protein was studied by traditional alkali extraction method,ultrasonic assisted method,and pulsed electric field assisted method,respectively.The law and mechanism of increasing the extraction efficiency of selenium-enriched soybean protein by physical field treatment were explored by mass transfer kinetic model.Ultrasound and pulsed electric fields promote protein dissolution through cavitation bubbles and electroporation effects,which in turn increase the extraction rate constant in the rapid extraction phase.Under the optimal conditions,the protein extraction amount of the three extraction methods reached equilibri-um at 120,60,and 90 min,respectively.The selenium-enriched soybean protein extraction rates of ultrasonic and pulsed electric field as-sisted extraction were 57.1%and 34.7%higher than those of traditional alkali extraction,respectively.And the ultrasound-assisted ex-traction of the protein had the highest total selenium content of 2.57 mg/kg.The structural properties of ordinary/selenium-enriched soy-bean proteins obtained by the three preparation methods were further analyzed.Results showed that ultrasound and pulsed electric field in-creased the fluorescence intensity and the surface hydrophobicity of the proteins,while selenium was opposite.The physical field treatment significantly increased the free sulfhydryl content and reduced the total sulfhydryl content.The contents of total amino acids and essential a-mino acids increased significantly.The secondary structure was altered(significantly increased α-helix and β-turn contents,reduced β-sheet contents),but no significant effect of selenium was observed on secondary structure.关键词
富硒大豆/蛋白/传质动力学/结构性质Key words
selenium-enriched soybean/protein/mass transfer kinetics/structural properties引用本文复制引用
张小芸,钟海琪,邵馨漫,李书艺,汪芳..富硒大豆蛋白的提取传质动力学及其结构性质研究[J].食品与发酵工业,2025,51(12):183-190,8.基金项目
湖北省自然科学基金计划项目(2024AFC027) （2024AFC027）
