食品与发酵工业2025，Vol.51Issue(12)：191-198,8.DOI:10.13995/j.cnki.11-1802/ts.039980
3-MPBA/AG@aZIF-7/PDA的制备及其对α-葡萄糖苷酶抑制肽的吸附
Preperation of 3-MPBA/AG@aZIF-7/PDA and their application in adsorption of α-glucosidase inhibitory peptides
摘要
Abstract
Immobilized enzymes have good stability and reusability compared to free enzymes and can be used for rapid screening of enzyme inhibitors.In this study,a novel immobilized α-glucosidase 3-MPBA/AG@aZIF-7/PDA was synthesized and characterized.In ad-dition,Ile-Tyr-Gly(ITG,non-competitive inhibitory peptide)and Cys-Tyr-Gly(CTG,competitive inhibitory peptide)were used as ad-sorption models to study the adsorption properties of 3-MPBA/AG@aZIF-7/PDA.Results showed that the adsorption capacity of 3-MPBA/AG@aZIF-7/PDA for ITG and CTG was 191.06 mg/g and 129.50 mg/g,respectively,under the optimized adsorption conditions.The adsorption kinetics and adsorption isotherm studies indicated that the adsorption of both ITG and CTG by 3-MPBA/AG@aZIF-7/PDA con-formed to the proposed one-stage adsorption kinetic model,which was mainly a physical adsorption process,and the adsorption process conformed to the Langmuir isothermal adsorption model that was dominated by the adsorption of monomolecular layers.Moreover,it was found that the immobilized enzyme could efficiently isolate the AG inhibitory peptides from the complex system.These results indicate that the immobilized enzyme is of great value in isolation and enrichment of functional peptides,and 3-MPBA/AG@aZIF-7/PDA is a promis-ing material for the efficient isolation of α-glucosidase inhibitors.关键词
α-葡萄糖苷酶/抑制肽/吸附动力学/吸附性能Key words
α-glucosidase/inhibitory peptides/adsorption kinetics/adsorption capacity引用本文复制引用
刘精杏,李焯颖,陈婷,黎彩霞,何子宇,王则奋,兰雄雕..3-MPBA/AG@aZIF-7/PDA的制备及其对α-葡萄糖苷酶抑制肽的吸附[J].食品与发酵工业,2025,51(12):191-198,8.基金项目
广西民族大学校引进人才科研启动项目(2018KJQD06) （2018KJQD06）
广西自然科学基金青年基金项目(2022GXNSFBA035499) （2022GXNSFBA035499）
广西自然科学基金面上项目(2021GXNSFAA075043) （2021GXNSFAA075043）
