食品与发酵工业2025，Vol.51Issue(12)：259-268,10.DOI:10.13995/j.cnki.11-1802/ts.040402
基于代谢组学研究不同通风比对普鲁兰多糖发酵的影响
Effects of different ventilation ratios on pullulan polysaccharide fermentation based on metabolomics
摘要
Abstract
In this paper,the intracellular metabolites of pullulan polysaccharide produced by Aureobasidium pullulans CGMCCNO.7055 were analyzed by GC-MS under different ventilation conditions using A.pullulans as the starting strain.It was found that ventilation had a significant effect on polysaccharide fermentation in pullulan,with the maximum polysaccharide yield of(80.2±1.4)g/L at high ventilation(1∶1.4)and a difference of 13.50 g/L between the lowest and the highest ventilation ratios in pullulan.The metabolites were analyzed in the fermentation of the high and low ventilation groups.A total of 45 intracellular small molecule metabolites were detected,mainly including amino acids,organic acids,sugars,alcohols,and some other classes of metabolites.The metabolic pathways of pullulan were combined with KEGG to analyze the differences in the metabolism of the fungus under different aeration conditions,and the differen-tial metabolites were mainly related to the pentose-glucuronide conversion pathway,fructose-mannose metabolism pathway,citric acid cyc-ling pathway,and galactose metabolism pathway,which ultimately led to changes in the production of pullulan polysaccharides.It provides a reference for further understanding of the metabolic mechanism study on the production of pullulan polysaccharides by germinating short ridge molds.关键词
出芽短梗霉/普鲁兰多糖/GC-MS/代谢组学/通风比Key words
Aureobasidium pullulans/pullulan polysaccharide/GC-MS/metabolomics/ventilation ratio引用本文复制引用
李淼鑫,马正旺,赵晓雯,李文敏,张伊凡,赵廷彬,乔长晟..基于代谢组学研究不同通风比对普鲁兰多糖发酵的影响[J].食品与发酵工业,2025,51(12):259-268,10.基金项目
宁夏回族自治区重点研发计划项目(2022BEG02006) （2022BEG02006）
