沈阳农业大学学报2025，Vol.56Issue(3)：61-75,15.DOI:10.3969/j.issn.1000-1700.2025.03.007
高通量测序分析向日葵—工业大麻轮作土壤微生物群落结构变化
High-throughput Sequencing Analysis of Soil Microbial Community Structure Changes in Sunflower-industrial Hemp Rotation
摘要
Abstract
[Objective]Continuous cropping obstacles can lead to soil degradation and crop yield reduction,which is one of the important factors limiting agricultural production.Reasonable crop rotation can alleviate the obstacles of continuous cropping.Industrial hemp is a suitable crop for rotation,which can maintain microbial diversity and increase biological yield.[Method]In this study,high-throughput sequencing technology was used to detect the dynamic changes of soil microbial diversity and community structure in sunflower and industrial hemp soils at different periods.[Results]A total of 45 phyla,143 classes,330 orders,552 families,1 160 genera and 1 795 species of bacteria were detected by high-throughput sequencing,among which Proteobacteria and Sphingosamonas were the most abundant phyla and genera,respectively.LEFSe analysis revealed that the biomarkers at 3 periods were Arthrobacter,Pyriformis,and Robacter,respectively.A total of 12 phyla,38 classes,79 orders,142 families,242 genera and 373 species of fungi were detected by high-throughput sequencing.Ascomycetes and Guehomyces were the most abundant phyla and genera,respectively.LEFSe analysis revealed that the biomarkers of 3 periods were Alternatispora,unidentified fungi,and Trichoderma,respectively.The diversity of microorganisms in the rhizosphere soil of sunflower and industrial hemp rotation was significantly different,and the inhibitory fungi in the rhizosphere soil of industrial hemp significantly increased,which inhibited the growth of pathogenic bacteria.[Conclusion]The results of this study provide a data foundation for exploring the mechanism of industrial hemp alleviating continuous cropping obstacles and the balance relationship between microbial crops.关键词
工业大麻/轮作/土壤微生物/高通量测序Key words
industrial hemp/crop rotation/soil microorganism/high-throughput sequencing分类
农业科学引用本文复制引用
张元野,张明,房郁妍,郑楠,闫博巍,隋月,张利国..高通量测序分析向日葵—工业大麻轮作土壤微生物群落结构变化[J].沈阳农业大学学报,2025,56(3):61-75,15.基金项目
黑龙江省农业科技创新跨越工程-经济作物突破性新品种选育及产业化应用项目(CX23GG04) （CX23GG04）
