沈阳农业大学学报2025，Vol.56Issue(3)：85-94,10.DOI:10.3969/j.issn.1000-1700.2025.03.009
外源2,4-表油菜素内酯对草莓采后抗坏血酸-谷胱甘肽循环的影响
Effect of 2,4-epibrassinolide Treatment on Ascorbic Acid-glutathione Cycle Metabolism in Strawberries
摘要
Abstract
[Objective]The ascorbic acid-glutathione(AsA-GSH)cycle metabolism,which participates in the scavenging of reactive oxygen species(ROS),is an important antioxidant system in plants,maintaining the redox balance of cells.Measuring the key metabolites and enzyme activities in the AsA-GSH cycle is of great significance for studying the antioxidant capacity of plants.2,4-epibrassinolide(EBR),which can promote plant cell growth and significantly enhance the resistance of plants to abiotic stress,is recognized as the sixth plant hormone.[Method]In this study,'Benihoppe'strawberries were used as the experimental material,and treated with 5 μmol·L-1 EBR spray.The effects of EBR treatment on the AsA-GSH cycle metabolism of strawberries during 5 days of storage at(20±1)℃were investigated.[Results]The results showed that EBR treatment could inhibit the occurrence of strawberry rot and the increase of malondialdehyde(MDA)content,delay the decline of total phenols,total anthocyanins content in strawberries,effectively inhibit the decrease of ascorbic acid(AsA),dehydroascorbic acid(DHA),reduced glutathione(GSH)and oxidized glutathione(GSSG)content,and increase the activities of glutathione reductase(GR),dehydroascorbate reductase(DHAR)and monodehydroascorbate reductase(MDHAR),as well as the expression levels of FaGR,FaDHAR and FaMDHAR genes.[Conclusion]Spraying 5 μmol·L-1 EBR reduces the degree of strawberries rot,thereby maintaining a higher content of antioxidant substances by regulating the AsA-GSH cycle system and alleviating the oxidative damage of postharvest strawberries.关键词
2,4-表油菜素内酯/草莓/抗坏血酸-谷胱甘肽循环/保鲜/品质Key words
2,4-epibrassinolide/strawberries/ascorbic acid-glutathione cycle/preservation/quality分类
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李美琳,孙文心,池慧敏,李兆星,纪淑娟,周鑫,罗曼莉,魏宝东,周倩..外源2,4-表油菜素内酯对草莓采后抗坏血酸-谷胱甘肽循环的影响[J].沈阳农业大学学报,2025,56(3):85-94,10.基金项目
辽宁省教育厅基本科研项目(JYTYB2024018) （JYTYB2024018）
