宋晓妍 1耿伟涛 1贾龙刚 1肖灵 1刘雅萱 1代欣佳 1石星雨 1师婧文 1韩雪梅 2孙韵琪 2王艳萍1
作者信息
- 1. 天津科技大学 食品科学与工程学院,天津,300457
- 2. 天津创源生物技术有限公司,天津,300301
- 折叠
摘要
Abstract
Constipation,a prevalent gastrointestinal ailment,primarily responds to medications like lubricating or stimulant laxatives;however,these treatments are often costly and accompanied by a risk of dependency.Consequently,there is a pressing demand for a viable alternative.Amidst the growing global focus on probiotics and their impact on human health,supplementing with probiotics has emerged as a significant therapeutic approach for constipation,aiming to harmonize intestinal flora and ultimately alleviate symptoms.Moreover,dark tea,renowned as the"purifier"of the digestive system,boasts an abundance of functional components like dietary fiber,polysaccharides,and vitamins.These elements collectively contribute to enhancing gastrointestinal motility,facilitating digestion,promoting urination,and acting as a natural laxative.Thus,dark tea presents itself as a promising adjunct in the quest for effective constipation management.In this study,two probiotic-infused dark teas,designated as BC and KC,were meticulously crafted by blending dark tea with laboratory-cultured Lactobacillus plantarum BC299 and commercially available probiotic formulations.Additionally,two distinct probiotic-fermented dark teas,BFC and KFC,were produced using dark tea through a fermentation process by BC299 or commercial probiotics.The primary objective of this investigation was to delve into the synergistic effects of dark tea,both in its original form and when combined with probiotics,on alle-viating constipation induced by loperamide hydrochloride in mice models.By analyzing these formulations,this study aimed to uncover po-tential therapeutic benefits and further the understanding of the interplay between traditional teas and modern probiotics in constipation.The findings revealed that the integration of probiotic dark tea and probiotic-fermented dark tea exhibited marked efficacy in enhancing the fecal water content and elevating the small intestinal transit rate among constipated mice.Furthermore,it significantly shortened the time to the first black stool expulsion in these animals.Notably,the combination also augmented the serum levels of motilin(MTL),gastrin(GAS),and tachykinin substance P(SP)in constipated mice,indicating a positive modulation of gut motility and digestive hormone pro-files.Concurrently,the fusion of probiotics and dark tea effectively addressed the harm inflicted on small intestinal tissue by constipation,prominently diminishing the concentration of inflammatory cytokines TNF-α and IL-1β within the intestinal tissue.Moreover,this combina-tion promoted a favorable inflammatory balance by augmenting the levels of the anti-inflammatory factor IL-10.Among the various formula-tions tested,the comprehensive assessment revealed that the probiotics-infused dark tea BC demonstrated the most pronounced efficacy in ameliorating constipation.Insights gained from 16S rDNA sequencing further illuminated that the integration of probiotics and dark tea was capable of reshaping the intestinal microbiota landscape,notably augmenting the Firmicutes/Bacteroidetes ratio within the gut of constipa-ted mice.This shift was particularly evident in the proliferation of Lactobacillus,underscoring the potential of this combination in fostering a favorable gut microbial ecosystem.In conclusion,the synergize of probiotics with dark tea emerged as an effective strategy for mitigating loperamide hydrochloride-induced constipation in mice.This combined approach not only repaired intestinal barrier impairments but also exerted a modulatory influence on the composition and architecture of the intestinal microbiota,thereby contributing to a healthier gut envi-ronment.This study offers a novel research perspective and serves as a pivotal reference,not only broadening the horizons of probiotics re-search but also pioneering the development of innovative functional foods aimed at intervening in and mitigating constipation,thereby con-tributing significantly to the advancement of this field.关键词
便秘/黑茶/益生菌/肠道菌群Key words
constipation/dark tea/probiotics/intestinal flora
