食品与发酵工业2025，Vol.51Issue(12)：346-351,6.DOI:10.13995/j.cnki.11-1802/ts.041410
超高效液相色谱-串联质谱法测定动物源性食品中4-羟基百菌清残留
Determination of 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
An analytical method for the determination of 4-hydroxychlorothalonil residues in animal-derived food by solid phase ex-traction purification combined with ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was estab-lished in this study.1%acetic acid acetonitrile solution was used to extract the samples by oscillation.The samples were purified by neu-tral alumina solid phase extraction column,defatted with n-hexane,and separated by Waters BEH C18 column under a gradient elution program using methanol and 1 mmol/L ammonium acetate aqueous solution as mobile phase.Electrospray ionization source negative ion was used to detect which mode was multiple reaction monitoring mode.The external standard method was selected to quantify.Results showed that the linear relationship of 4-hydroxychlorothalonil was good in the range of 5-500 μg/L when the correlation coefficient(r2)was0.999 4.The limit of quantitation was determined to be0.005 mg/kg.When pork,beef,mutton,chicken,chicken skin,pork loin,lard,chicken liver,and milk were used as the substrates,the average recoveries were between76.9%and104.7%under three different addition levels.At the same time,the relative standard deviations of 4-hydroxychlorothalonil were 0.6%-4.6%.The pretreatment method is simple and easy to operate,the result is not only accurate but also reliable.Therefore,this analytical method is suitable for the quantita-tive determination of 4-hydroxychlorothalonil in animal-derived food,which is helpful for the market supervision of 4-hydroxychlorothalonil residues in animal-derived food.关键词
超高效液相色谱-串联质谱法/4-羟基百菌清/动物源性食品/固相萃取Key words
ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)/4-hydroxychlorothalonil/ani-mal-derived food/solid phase extraction引用本文复制引用
刘小芳,蒋军杰,石鹏途,何浩,田雄,郭丹,李欣,陈媛媛..超高效液相色谱-串联质谱法测定动物源性食品中4-羟基百菌清残留[J].食品与发酵工业,2025,51(12):346-351,6.基金项目
湖南省自然科学基金科市联合项目(2023JJ60530) （2023JJ60530）
