中国食品添加剂2025，Vol.36Issue(6)：13-20,8.DOI:10.19804/j.issn1006-2513.2025.6.003
超声联合酶辅助蒸馏法优选秦岭艾草挥发油提取工艺及其抗氧化活性研究
Optimization of the extraction technology of volatile oil from Qinling Artemisia argyi by ultrasound combined with enzyme-assisted distillation and its antioxidant activity
摘要
Abstract
Based on a single-factor experiments,under the conditions of a fixed ultrasonic power of 100 W,a solid-to-liquid ratio of(1∶20)g/mL and an enzymatic hydrolysis time of 60 min,the response surface method was used to optimize the extraction process of volatile oil from Qinling Artemisia argyi by ultrasonic combined with enzyme-assisted distillation,and the antioxidant activity was assessed by scavenging DPPH and ABTS+free radicals.The results showed that when the extraction time was 4.3 h,the enzymatic hydrolysis temperature was 54℃,the enzyme amount was 125 U/g,and the ultrasonic time was 47 min,the extraction rate of volatile oil from Qinling Artemisia argyi was 0.7549％,which was 0.0749％lower than the predicted value by the software.The results of antioxidant test showed that when the concentration of volatile oil was 1.0 mg/mL,the scavenging rates of DPPH and ABTS+free radicals were 91.83％and 92.53％,respectively,which were close to the scavenging rates of positive control Vc at the same concentration(94.48％and 95.32％),but there was no significant difference(P＞0.05).The clearance rate increased with the in-crease of concentration.This study provides a reference for the development and utilization of Artemisia argyi resources in Qinling Mountains.关键词
超声联合酶辅助蒸馏法/秦岭艾草/挥发油提取/抗氧化活性Key words
ultrasonic combined with enzyme-assisted distillation method/Artemisia argyi from Qinling mountains/volatile oil extraction/antioxidant activity分类
轻工业引用本文复制引用
侯敏娜,李丹,侯少平,豆佳媛,周鹏韬,赖普辉..超声联合酶辅助蒸馏法优选秦岭艾草挥发油提取工艺及其抗氧化活性研究[J].中国食品添加剂,2025,36(6):13-20,8.基金项目
陕西省中药质量标志物创新团队(SSY18TD02) （SSY18TD02）
2024年陕西省科技厅计划项目(2024SF-YBXM-474) （2024SF-YBXM-474）
2023年陕西省教育厅计划项目(23JK0326) （23JK0326）
