食品与机械2025，Vol.41Issue(6)：1-8,8.DOI:10.13652/j.spjx.1003.5788.2025.80274
代谢工程改造酿酒酵母从D-木糖合成柚皮苷
Metabolic engineering of Saccharomyces cerevisiae for de novo biosynthesis of naringin from D-xylose
摘要
Abstract
[Objective]To explore the biosynthesis method of naringin,a bioactive compound in citrus.[Methods]Engineered Saccharomyces cerevisiae strains for naringenin biosynthesis are constructed through enzyme screening,multi-copy integration,and precursor supply.Subsequently,de novo biosynthesis of naringin from D-xylose is achieved by introducing the naringin biosynthetic pathway and optimizing UDP-glucose supply.[Results]The optimal flavonoid synthase is identified as PhCHS by screening the key enzymes of the naringenin pathway.The naringenin biosynthesis pathway is further integrated at multi-copy sites.The constructed strain Z39M2 can synthesize 36.53 mg/L naringenin.The overexpression of CAT2,xPK-PTA pathway,and ACC1m optimizes malonyl-CoA supply and constructs strain Z44,with a naringenin production increasing to 72.96 mg/L.Finally,the naringin biosynthesis pathway introduction enables the successful biosynthesis of 35.60 mg/L naringin.Further overexpression of PGM1 and UGP1 elevates naringin production to 76.67 mg/L.[Conclusion]An engineered S.cerevisiae strain capable of de novo biosynthesis of naringin from D-xylose is successfully constructed by metabolic engineering.关键词
D-木糖/柚皮苷/柚皮素/酿酒酵母/代谢工程Key words
D-xylose/naringin/naringenin/Saccharomyces cerevisiae/metabolic engineering引用本文复制引用
赵逸飞,李高阳,黄绿红,刘娟,肖志强..代谢工程改造酿酒酵母从D-木糖合成柚皮苷[J].食品与机械,2025,41(6):1-8,8.基金项目
岳麓山实验室联合引进人才项目(编号:2024RC2055) （编号:2024RC2055）
洞庭实验室专项经费项目(编号:2024-DTZD-001) （编号:2024-DTZD-001）
