食品与机械2025，Vol.41Issue(6)：36-43,8.DOI:10.13652/j.spjx.1003.5788.2025.80170
UPLC-MS/MS柱前衍生对食用菌中麦角甾醇含量的影响
Effect of UPLC-MS/MS pre-column derivatization on ergosterol content in edible fungi
摘要
Abstract
[Objective]To improve the responsiveness of ergosterol in electrospray ionization mode and to investigate the ergosterol derivatization through dansylation.[Methods]With dansyl chloride as the derivatizing agent and 4-dimethylaminopyridine(DMAP)as the catalyst,this paper carries out a response surface test to optimize derivatization conditions.The samples are separated on a C18 column utilizing isocratic elution with a 0.15%formic acid-methanol solution as the mobile phase.Multiple reaction monitoring is performed using an ESI source.The ergosterol content in 10 edible fungi is assessed.[Results]The optimal derivatization conditions are the reaction temperature of 36℃,the reaction time of 25 min,and the DMAP mass concentration of 8 µg/mL.The derivatized ergosterol exhibits a broad linearity range of 25～2 000 ng/mL.The detection limit is 13.4 ng/mL,while the quantification limit is 149 ng/mL,with the sensitivity 51.94 times higher than the pre-derivatization state.Among the 10 edible fungi,shiitake mushrooms exhibit the highest ergosterol content,which is(4.21±0.083)mg/g.[Conclusion]This method is characterized by its ease of operation,high sensitivity,and accuracy.关键词
麦角甾醇/衍生化/丹磺酰化/超高效液相色谱—质谱联用/食用菌Key words
ergosterol/derivatization/dansylation/ultra-high performance liquid chromatography-tandem mass spectrometry/edible fungi引用本文复制引用
张新月,陈杭君,牛犇,申屠旭萍,杨海龙,殷明,郜海燕,穆宏磊..UPLC-MS/MS柱前衍生对食用菌中麦角甾醇含量的影响[J].食品与机械,2025,41(6):36-43,8.基金项目
国家自然科学基金(编号:32172282) （编号:32172282）
浙江省高层次人才特殊支持计划(编号:2022R52025) （编号:2022R52025）
国家重点研发计划课题(编号:2021YFD2100502) （编号:2021YFD2100502）
