食品工业科技2025，Vol.46Issue(13)：207-217,11.DOI:10.13386/j.issn1002-0306.2024030488
6'-O-咖啡酰熊果苷脂质体的制备工艺优化及其活性研究
Optimization of the Preparation Process of 6'-O-Caffeoylarbutin Liposomes and Its Activity
摘要
Abstract
Objective:To optimize the preparation process of 6'-O-caffeoylarbutin liposomes using the Box-Behnken response surface methodology,and to characterize their structure and evaluate their activity.Methods:The ethanol injection method was used.Initially,single-factor experiments were conducted to investigate the effects of the mass ratio of soybean lecithin to cholesterol,the pH of phosphate-buffered saline(PBS),the amount of 6'-O-caffeoylarbutin,stirring temperature,and stirring time on the encapsulation efficiency of 6'-O-caffeoylarbutin liposomes.Based on the results of single-factor experiments,the Box-Behnken response surface methodology was employed to optimize the preparation process.The liposomes were then characterized in terms of microscopic morphology,particle size,in vitro antioxidant activity,and in vitro tyrosinase inhibition activity.Results:The optimal conditions for preparing liposomes were found to be a soybean lecithin to cholesterol mass ratio of 7.10(g/g),a PBS pH of 6.90,an amount of 820 μL of 6'-O-caffeoylarbutin,and stirring at 45 ℃ for 30 minutes,resulting in a maximum encapsulation efficiency of 80.93％±0.82％.The obtained liposomes exhibited a vesicular structure,with a nearly spherical morphology.The particle size ranged primarily from 78 to 200 nm,with an average size of 127.42±0.76 nm and a polydispersity index of 0.259±0.006.The Zeta potential mainly ranged from-80 and-40 mV.The IC50 values for 6'-O-caffeoylarbutin and its liposomes in scavenging DPPH radicals were 0.27±0.04 and 0.26±0.03 mg/mL,respectively.For ABTS+radicals,the IC50 values were 0.13±0.02 mg/mL and 0.17±0.03 mg/mL,and for OH radicals,the IC50 values were 0.10±0.03 and 0.17±0.02 mg/mL.The total reducing power for iron ions was significantly higher for 6'-O-caffeoylarbutin than for its liposomes.The maximum inhibition rates of the liposomes against tyrosinase monophenolase activity were 48.81％±0.51％and 42.56％±1.25％,with IC50 values for diphenolase inhibition of 0.14±0.02 and 0.16±0.03 mg/mL,respectively.Conclusion:The liposomes prepared under the experimental conditions exhibited high encapsulation efficiency and small particle size for 6'-O-caffeoylarbutin,demonstrating good in vitro antioxidant activity and tyrosinase inhibition.This study provides a reference for the deep development and utilization of 6'-O-caffeoylarbutin.关键词
6'-O-咖啡酰熊果苷/脂质体/包封率/抗氧化/酪氨酸酶抑制Key words
6'-O-caffeoylarbutin/liposomes/encapsulation efficiency/antioxidant/tyrosinase inhibition分类
轻工纺织引用本文复制引用
刘梦哲,韩康佳,王文博,杨洋,解思达,赵平,徐俊明,何远平,杨晓琴..6'-O-咖啡酰熊果苷脂质体的制备工艺优化及其活性研究[J].食品工业科技,2025,46(13):207-217,11.基金项目
国家自然科学基金(32060327,32060107,22267018) （32060327,32060107,22267018）
云南省中青年学术和技术带头人后备人才项目(202205AC160049) （202205AC160049）
云南省科技厅基础研究计划(202401AT070295) （202401AT070295）
云南省教育厅科学研究基金(2023Y0698) （2023Y0698）
云南省兴滇人才计划 （）
昆明远方生物制品有限公司赵平专家基层科研工作站. （）
