食品工业科技2025，Vol.46Issue(13)：218-232,15.DOI:10.13386/j.issn1002-0306.2024060044
超声辅助低共熔溶剂提取欧李原花青素工艺优化及其生物活性
Optimization of Ultrasound-assisted Deep Eutectic Solvent Extraction Process and Biological Activities of Cerasus humilis Proanthocyanidin
摘要
Abstract
This study aimed to optimise the ultrasound-assisted deep eutectic solvent extraction of Cerasus humilis proanthocyanidin(CPC)and to compare it with the traditional 70％ethanol extraction method,focusing on the assessment of the structural composition and biological activity of the purified materials.The extraction process for ultrasound-assisted deep eutectic solvent extraction of CPC was optimised using single-factor combined response surface methodology,the average degree of polymerisation was determined after purification,and the functional groups and composition of CPC were analysed qualitatively by Fourier infrared spectroscopy and high performance liquid tandem mass spectrometry(UPLC-OE-MS/MS).The effects of temperature,light,metal ions and pH on the stability of CPC were explored,and the antioxidant capacity of CPC was assessed with the help of DPPH,ABTS+free radical scavenging assay and FRAP method,and the in vitro hypoglycaemic activity of CPC was investigated by α-amylase and α-glucosidase inhibition assays.The results showed that choline chloride-1,2 propanediol(molar ratio 2∶1)was screened as the extraction solvent,and the yield of CPC was 21.11％±0.10％,which was 1.5 times higher than that of 70％ethanol extraction,at 40％(V/V)water content,1∶20(g/mL)material-liquid ratio,49 ℃ ultrasonication temperature,and 49 min extraction time.The purified purity of DES-extracted CPC was 87.41％±2.54％,and the average degree of polymerisation was 3.09±0.02.CPC was based on protocyanin as the main structural unit and oligomeric protocyanidins as the main components.The retention rate of CPC was more than 85％after 10 h at 20 ℃ protected from light,however,the stability was significantly reduced under high temperature,alkaline and light conditions,and it was greatly damaged by metal ions Fe3+,Fe2+and Cu2+.In addition,CPC showed strong antioxidant properties and hypoglycemic activity in vitro.In conclusion,the ultrasound-assisted deep eutectic solvent extraction method not only significantly improved the extraction yield and purity of CPC,but also performed excellently in maintaining its bioactivity,which provided an important theoretical basis for the green extraction and application of functional active substances in Cerasus humilis.关键词
欧李原花青素/低共熔溶剂/高效液相串联质谱(UPLC-OE-MS/MS)/稳定性/抗氧化/降血糖Key words
Cerasus humilis proanthocyanidin/deep eutectic solvent/UPLC-OE-MS/MS/stability/antioxidant/hypoglycemic分类
轻工纺织引用本文复制引用
赵悦,褚天旭,高哲,赵文,孙亚赛..超声辅助低共熔溶剂提取欧李原花青素工艺优化及其生物活性[J].食品工业科技,2025,46(13):218-232,15.基金项目
河北农业大学引进人才科研专项(YJ2020029) （YJ2020029）
河北省高等学校基本科研业务费(KY2023045). （KY2023045）
