食品与机械2025，Vol.41Issue(6)：88-95,8.DOI:10.13652/j.spjx.1003.5788.2024.80973
无标记适配体—金属有机框架模拟酶比色检测牛奶和奶粉中双酚A
Colorimetric detection of bisphenol A in milk and milk powder based on label-free aptamer and metal-organic framework enzyme mimics
摘要
Abstract
[Objective]To develop a label-free and visual colorimetric assay for the rapid detection of bisphenol A(BPA)using a BPA aptamer as the recognition molecule and metal-organic framework(Fe-MIL-101)nanozyme mimics as the signal probe.[Methods]The BPA aptamer was added to the Fe-MIL-101 solution,where it was adsorbed onto the Fe-MIL-101 surface through electrostatic interactions,thereby inhibiting the activity of nanozyme mimics.In the presence of BPA in the test sample,the aptamer specifically binds to BPA and detaches from the Fe-MIL-101 surface,restoring the catalytic activity of Fe-MIL-101.This enables Fe-MIL-101 to catalyze a colorless peroxidase substrate,generating a colorimetric output signal.The qualitative and quantitative analysis of BPA was achieved by monitoring the changes in the colorimetric signal.[Results]Under optimized conditions,the linear detection range for BPA in a buffered solution was 1～80 ng/mL,with a naked-eye visual detection limit of 0.5 ng/mL.When applied to BPA detection in milk and milk powder,the method achieved spike recoveries ranging from 83.6%to 95.2%,with relative standard deviations(RSDs)between 2.96%and 7.45%.[Conclusion]This method is simple,rapid,and highly accurate,demonstrating strong potential for practical applications.关键词
双酚A/适配体/金属有机框架/无标记/比色分析Key words
bisphenol A/aptamer/metal-organic framework/label-free/colorimetric assay引用本文复制引用
姜黎,卢春霞,林祥群,陈霞..无标记适配体—金属有机框架模拟酶比色检测牛奶和奶粉中双酚A[J].食品与机械,2025,41(6):88-95,8.基金项目
重庆市教委科学技术研究计划重点项目(编号:KJZD-K202201405) （编号:KJZD-K202201405）
