食品工业科技2025，Vol.46Issue(13)：404-412,9.DOI:10.13386/j.issn1002-0306.2024070134
具有转糖基活性几丁质酶和壳聚糖酶的研究进展
Research Progress on Chitinase and Chitosanase with Transglycosylation Activity
摘要
Abstract
N-acetyl chitooligosaccharides and chitooligosaccharides with high degree of polymerization are highly valued for their superior bioactivities.However,traditional preparation methods face various limitations,such as uncontrollable processes and challenges inseparation and purification.Recent studies have discovered that certain chitinases and chitosanases possess both hydrolysis and transglycosylation(TG)activities.The TG reaction offers advantages in producing oligosaccharides with high-degree polymerization,including easy accessibility to substrates and low production costs.This paper reviews recent advances in chitinases and chitosanases with TG activity,analyzes the key amino acid residues affecting TG activity in chitinases,and discusses the impact of molecular modifications of these key residues on TG activity.The goal is to provide insights for further research on chitinases and chitosanases with TG activity and their subsequent development and utilization,thereby promoting the green production and industrialization of oligosaccharides with high-degree polymerization.关键词
转糖基活性/几丁质酶/壳聚糖酶/高聚合度/分子改造Key words
transglycosylation activity/chitinase/chitosanase/high deep of polymerization/molecular modification分类
生物科学引用本文复制引用
未莹莹,桂敏娟,孙慧慧,赵玲,曹荣..具有转糖基活性几丁质酶和壳聚糖酶的研究进展[J].食品工业科技,2025,46(13):404-412,9.基金项目
山东省重点研发计划(2023TZXD052). （2023TZXD052）
