食用菌学报2025，Vol.32Issue(3)：120-130,11.DOI:10.16488/j.cnki.1005-9873.2025.03.012
野生白灵侧耳种质资源遗传多样性分析和评价
Genetic Diversity Analysis and Evaluation of Wild Pleurotus tuoliensis Germplasm Resources
摘要
Abstract
Fifty two wild Pleurotus tuoliensis strains from Tacheng area of Xinjiang province were analyzed for genetic diversity using ISSR markers.They were also observed and measured for agronomic traits(five qualitative and ten quantitative traits),and then evaluated based on the quantitative traits.For genetic diversity analysis,the genomic DNA of the 52 strains were extracted from their mycelia,amplified for ISSR markers,measured for number of alleles,number of polymorphic loci,percentage of polymorphic loci,and number of effective alleles,and calculated for Shannon diversity index and Nei's gene diversity index.Then a dendrogram of the ISSR sequences of the 52 strains of P.tuoliensis was constructed using the unweighted pair group method with arithmetic mean(UPGMA)algorithm.For the quantitative traits,Q-cluster analysis and principal component analysis were carried out by SPSS 16.The results showed that a total of 64 polymorphic loci were amplified by 10 ISSR primers,and the average Nei's gene diversity index was 0.25.The wild P.tuoliensis strains were categorized into five clusters by UPGMA cluster analysis,and the genetic similarity coefficients were between 0.63 and 0.93.Shannon diversity index for the five qualitative traits ranged from 1.01 to 1.26,and that for the 10 quantitative traits ranged from 1.82 to 2.05,with the largest diversity index of 2.05 for stipe diameter.When the squared Euclidean distance of the Q-cluster analysis of the quantitative traits was 15,the 52 strains were categorized into three groups,including strain JZB2106018 in group 1,strain JZB2106020 in group 2,and the remaining 50 strains in group 3.There was a rich genetic diversity within the 52 strains.Based on the eigenvalues,eigenvectors of each principal component and the standardized quantitative trait data,comprehensive scores were calculated for each strain,and three strains,JZB2106048,JZB2106045 and JZB2106039,showed a high comprehensive score.The single fruiting body weights of the three strains were also relatively large.They can be used as breeding materials or high-quality germplasm resources for P.tuoliensis.关键词
野生白灵侧耳/种质资源/农艺性状/遗传多样性/主成分分析Key words
Wild Pleurotus tuoliensis/germplasm resource/agronomic trait/genetic diversity/principal components analysis引用本文复制引用
宋爽,刘宇,武月园,高琪,吴灵芝,张欣媚,王守现..野生白灵侧耳种质资源遗传多样性分析和评价[J].食用菌学报,2025,32(3):120-130,11.基金项目
北京市农林科学院科技创新能力建设专项(KJCX20230102) （KJCX20230102）
国家现代农业产业技术体系(CARS-20) （CARS-20）
