食品与机械2025，Vol.41Issue(6)：157-163,7.DOI:10.13652/j.spjx.1003.5788.2025.80157
文冠果油促进斑马鱼血管再生的分子机制研究
Molecular mechanisms underlying Xanthoceras sorbifolium oil-promoted vascular regeneration in zebrafish
摘要
Abstract
[Objective]To investigate the effects of Xanthoceras sorbifolium oil(XSO)in promoting vascular regeneration in zebrafish and its mechanisms,and to provide a basis for medicine development in cardiovascular repair.[Methods]A PTK787-induced vascular defect model is built in zebrafish.XSO is administered at concentrations of 0,25,50,75,and 100 μg/mL.The regeneration of intersegmental vessels(ISV)and subintestinal vessels(SIV)is analyzed via fluorescence microscopy.Oxidative stress markers(SOD,MDA,CAT)and Apela protein expression levels are detected.Additionally,DHI(Danhong Injection)is set as a positive control.[Results]The 75 μg/mL XSO group shows optimal efficacy in vascular regeneration promotion:ISV length recovers to 87.6%of the normal group(2 234.5 μm vs.2 550.1 μm),and SIV length exceeds normal levels by 105%(662.8 μm vs.627.9 μm),outperforming the positive control group(ISV:93.7%;SIV:98.2%).Oxidative stress indexes are significantly alleviated:SOD is 19.03 U/mg(1.41-fold of the normal group),MDA content is decreased to 56.6%of the model group(1.305 nmol/mg vs.the positive control group 1.658 nmol/mg),and CAT activity reaches 1.623 4 U/mL(1.53-fold of the normal group,1.32-fold of the positive control group).Apela protein expression is at 49.327 ng/mL(1.26-fold of the model group vs.1.18-fold of the positive control group).[Conclusion]XSO promotes vascular regeneration by modulating oxidative stress and upregulating Apela protein,demonstrating superior efficacy and safety to DHI without excessive risks of vascular regeneration.关键词
文冠果油/斑马鱼/血管再生/氧化应激/Apela蛋白Key words
Xanthoceras sorbifolium oil/zebrafish/vascular regeneration/oxidative stress/Apela protein引用本文复制引用
陈海燕,贾爱玲,刘纪盈,王超,邱智东..文冠果油促进斑马鱼血管再生的分子机制研究[J].食品与机械,2025,41(6):157-163,7.基金项目
长春科技学院科学基金项目(编号:2024ZD001) （编号:2024ZD001）
