食用菌学报2025，Vol.32Issue(3)：28-37,10.DOI:10.16488/j.cnki.1005-9873.2025.03.003
刺芹侧耳苯丙氨酸解氨酶基因表达和酶活性与菌盖颜色相关性
Analysis of Phenylalanine Ammonia-Lyase Gene Expression and Its Effect on Pileus Color in Pleurotus eryngii
摘要
Abstract
Pleurotus eryngii strain Pe0100 was analyzed for phenylalanine ammonia-lyase(PAL)gene sequences,and expression pattern of PAL genes across five developmental stages,mycelium,primordium,young mushroom,elongation,and maturation.In particular,the expression of PAL genes in pileus and stipe was compared at the young mushroom,elongation and maturation stages.Using Pe0100 as the control,one of the PAL genes in P.eryngii,PePAL1,was overexpressed in Pe0100 by transformation of the overexpression plasmid Pe-PAL1.The PePAL1 overexpression transformants were then observed for phenotypic characteristics and analyzed for correlation between PAL activity and PAL gene expression in mature pileus.The results showed that there were two PAL genes,PePAL1 and PePAL2,in the genome of Pe0100.Compared with stipe,pileus showed higher expression of PePAL1 at the young mushroom,elongation and maturation stages.On the other hand,PePAL2 showed a higher expression level than PePAL1 in mycelium,and remained a stable expression level during the rest of the developmental stages.Four PePAL1 overexpression transformants,OE#3,OE#10,OE#16 and OE#18,were obtained.All of them produced dark brown striped pigmentation on the surface of their pilei,and there was a positive correlation between PePAL1 expression and PAL enzyme activity in mature pileus.These results provided a reference for breeding of new P.eryngii cultivars.关键词
过表达/基因表达量/菌盖/菌柄Key words
Overexpression/gene expression level/pileus/stipe引用本文复制引用
聂梦涵,陈莉莉,罗莉媛,张拓,谢宝贵..刺芹侧耳苯丙氨酸解氨酶基因表达和酶活性与菌盖颜色相关性[J].食用菌学报,2025,32(3):28-37,10.基金项目
国家重点研发计划(2022YFD1200604) （2022YFD1200604）
