食用菌学报2025，Vol.32Issue(3)：38-50,13.DOI:10.16488/j.cnki.1005-9873.2025.03.004
基于转录组分析高温对六妹羊肚菌菌丝体基因表达的影响
Transcriptome Analysis of Gene Expression in Morchella sextelata Mycelium under High-Temperature Stress
摘要
Abstract
Using 20 ℃ as the control,the effects of high-temperature(33 ℃)stress on gene expression in mycelia of Morchella sextelata were studied.Comparative transcriptome analysis was used to identify differentially expressed genes(DEGs)between the two experimental conditions.Five randomly selected DEGs were validated by real-time fluorescence quantitative PCR(qPCR),and the correlation between trasnscriptiome data and qPCR results was analyzed by Pearson correlation analysis.The identified DEGs were analyzed for gene ontology(GO)and KEGG metabolic pathway.Based on the screened metabolic pathways,gene set enrichment analysis(GSEA)was carried out to identify genes involved in high-temperature response of mycelia.The results showed that there were 2 112 DEGs between the high-temperature group and the control group.Among them,799 genes were up-regulated,and 1 313 genes were down-regulated.There was a highly significant positive correlation(R2=0.88)between the qPCR results and the transcriptome sequencing data of the five randomly selected DEGs.The GO functional enrichment analysis showed that the DEGs were primarily enriched in DNA replication,lipid metabolism,membrane components,DNA binding,and drug binding.The KEGG enrichment analysis showed that the DEGs were enriched in several crucial metabolic pathways,such as mismatch repair,ABC transporter,starch and sucrose metabolism,pyruvate metabolism,amino acid biosynthesis,two-component system,RNA degradation,and carbon metabolism.Ten key DEGs were identified to be associated with high-temperature stress,and they were genes encoding DNA helicase,molecular chaperone protein,pyruvate kinase,malolactic enzyme,ATP-binding protein,multidrug resistance efflux pump protein,phosphodiesterase,histidine kinase,transketolase,and fructose-6-phosphate ketolase.These findings provided a reference for elucidating the molecular mechanism underlying response of M.sextelata to high-temperature stress.关键词
六妹羊肚菌/高温胁迫/转录组测序/基因表达Key words
Morchella sextelata/high-temperature stress/transcriptome sequencing/gene expression引用本文复制引用
胡鑫钰,翟梦丹,陈泽威,李汪麒,黄凯文,张宇辰,王震..基于转录组分析高温对六妹羊肚菌菌丝体基因表达的影响[J].食用菌学报,2025,32(3):38-50,13.基金项目
黄淮学院国家级科研项目培育基金(XKPY-2022008) （XKPY-2022008）
驻马店市重点研发专项(ZMD52DZX2023010) （ZMD52DZX2023010）
河南省食用菌产业技术体系(HARS-22-08-Z5) （HARS-22-08-Z5）
