食用菌学报2025，Vol.32Issue(3)：51-58,8.DOI:10.16488/j.cnki.1005-9873.2025.03.005
六妹羊肚菌几个发育阶段细胞核变化
Changes of Nuclei in Morchella sextelata at Different Developmental Stages
摘要
Abstract
Morchella sextelata was observed for nuclear morphology of ascus,ascospore,primary hypha and secondary hypha by fluorescence microscopy.The results showed that initially there were 10-24 nuclei per ascus,which then moved to the tip and gathered.After cell division,eight oval-shaped ascospores were formed and arranged vertically in a column per ascus.After ascospores were ejected from asci,their volume doubled after cultured for 6 h,and the number of nuclei increased from 9-15 per ascospore to about 21-27 per ascospore.As the cultivation continued,ascospore tip bulged and developed 1-2 germ tubes,which then further elongated and developed septa.There were both single mating type and double mating type in primary hyphae,whereas secondary hyphae were exclusively double mating type.Under microscopic observation,there were differences in nuclear morphology between primary hyphae and secondary hyphae.Primary hyphae typically held 25-52 nuclei per cell,and the nuclei were predominantly granular and small.On the other hand,secondary hyphae typically held 3-10 nuclei per cell,and the nuclei were mostly clumpy and large.Regardless of nuclear shape,both granular and clumpy nuclei were able to transport genetic material between cells through septum.关键词
羊肚菌/子囊孢子/菌丝/细胞核Key words
Morchella/ascospore/hypha/nucleus引用本文复制引用
王晓敏,张钦语,樊扬,张邦喜..六妹羊肚菌几个发育阶段细胞核变化[J].食用菌学报,2025,32(3):51-58,8.基金项目
国家重点研发计划项目(2022YFD1601704) （2022YFD1601704）
贵州省科技支撑计划项目(黔科合支撑[2022]一般083、黔科合支撑[2023]一般054) （黔科合支撑[2022]一般083、黔科合支撑[2023]一般054）
国家食用菌产业技术体系(CARS-20) （CARS-20）
贵州省食用菌产业技术体系(GZMARS-SYJ-2024-2026) （GZMARS-SYJ-2024-2026）
