食用菌学报2025，Vol.32Issue(3)：91-101,11.DOI:10.16488/j.cnki.1005-9873.2025.03.009
采用振荡-静置循环培养策略制备灵芝菌丝体的水提物抗氧化活性
Antioxidant Activity of Aqueous Extract of Ganoderma lucidum Mycelia Prepared under a Shaking-Static Cultivation Cycle
摘要
Abstract
Using a shaking-static cultivation cycle of 2.8 d shaking-7.3 d static-0.2 d shaking-0.3 d static,mycelia of Ganoderma lucidum were cultivated in 250 mL shake flasks,3 L,5 L,and 50 L bioreactors,respectively.At the same time,continues shaking cultivation at these scales were set as controls.Then mycelia harvested from different cultivation scales were measured for biomass and total triterpene content,and aqueous extracts of mycelia harvested from different cultivation scales were measured for contents of total triterpenes,total phenols,and total sugar.The aqueous extracts were also determined for their activities on scavenging ABTS,DPPH,superoxide anion,and hydroxyl radicals.The results showed that mycelia of shaking-static cultivation groups contained higher amount of total triterpenes compared with the control groups.Under the shaking-static cultivation mode,cultivation in bioreactors achieved higher biomass than cultivation in 250 mL shake flasks,while cultivation at 250 mL scale resulted in the highest total triterpene content among all scales.For aqueous extracts of mycelia obtained under the shaking-static cultivation mode,the aqueous extract of 3 L bioreactor cultivated mycelia contained the highest contents of total triterpenes and total sugar,whereas the aqueous extract of 250 mL shake flask cultivated mycelia and that of 3 L bioreactor cultivated mycelia contained high levels of total phenols.In terms of antioxidant activity in vitro,the aqueous extract of 3 L bioreactor cultivated mycelia showed the strongest ABTS radical scavenging activity[IC50(12.31±0.23)mg·mL-1],while the aqueous extract of 250 mL shake flask cultivated mycelia exhibited the highest activity on scavenging DPPH radicals[IC50(4.53±0.24)mg·mL-1].For superoxide anion radicals,the aqueous extracts of mycelia from 3 L and 50 L bioreactors showed relatively strong scavenging activity,with IC50 of(12.29±0.61)mg·mL-1 and(12.66±0.68)mg·mL-1,respectively.For hydroxyl radicals,the aqueous extracts of mycelia from 250 mL shake flasks,5 L,and 50 L bioreactors showed strong scavenging activity,with IC50 of(12.52±0.73)mg·mL-1,(11.87±0.46)mg·mL-1,and(11.57±0.33)mg·mL-1,respectively.These findings provided a reference for industrialized production and applications of G.lucidum mycelia by liquid fermentation.关键词
灵芝三萜/抗氧化活性/液态发酵/振荡-静置循环培养策略Key words
Ganoderma lucidum triterpenes/antioxidant activity/liquid fermentation/shaking-static cyclic fermentation strategy引用本文复制引用
王星,冯杰,姜幸怡,刘艳芳,韩伟,张劲松..采用振荡-静置循环培养策略制备灵芝菌丝体的水提物抗氧化活性[J].食用菌学报,2025,32(3):91-101,11.基金项目
上海市现代农业产业技术体系(沪农科产字[2022]第9号) （沪农科产字[2022]第9号）
上海市农业科学院卓越团队建设计划(沪农科卓[2022]003) （沪农科卓[2022]003）
