现代食品科技2025，Vol.41Issue(6)：286-297,12.DOI:10.13982/j.mfst.1673-9078.2025.6.0229
基于UPLC-MS/MS法对17种罗汉果苷含量的测定
Determination of 17 Mogrosides by UPLC-MS/MS
摘要
Abstract
Mogrosides are a class of tetracyclic triterpenoid saponins found predominantly in Siraitia grosvenorii(Luo Han Guo),a fruit widely used in traditional Chinese medicine.In this study,an efficient and rapid detection method was developed to detect 17 kinds of mogrosides in S.grosvenorii using ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry(UPLC-MS/MS)combined with targeted metabolomics and their variations during fruit development.The results demonstrated that the 17 mogrosides exhibited good linearity(R>0.990)within their detection ranges,with the average spiked recovery rate exceeding 95%.In the different linear ranges of the mogrosides,detection limits were between 0.047 and 3.834 μg/L,while quantitation limits were between 0.158 and 12.780 μg/L.At the early fruit growth stage(15 to 30 d)of S.grosvenorii,mogroside IIe was the predominant mogroside that continued to accumulate.At the middle growth stage(30 to 50 d),mogroside III and other mogrosides gradually accumulated.As the fruit continued to grow,these mogrosides transformed into various compounds,such as siamenoside I.At 50 to 70 d,compounds such as siamenoside I glycosylated to generate mogrosides,including mogroside V.In the final growth stage(70 to 90 d),saponin accummulation mainly consisted of mogroside V,generated through glycosylation,which reached its peak and then stabilized.The proposed method offers an ideal approach for qualitative and quantitative analysis of multiple isomers of mogrosides in S.grosvenorii samples,providing high sensitivity and accuracy.关键词
罗汉果/罗汉果苷/超高效液相色谱串联质谱/四环三萜皂苷Key words
Siraitia grosvenorii/mogrosides/ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry/triterpenoid saponins引用本文复制引用
王哲斐,裴继影,卢歆怡,李检秀,谢能中,蒋水元,蒙健宗..基于UPLC-MS/MS法对17种罗汉果苷含量的测定[J].现代食品科技,2025,41(6):286-297,12.基金项目
国家自然科学基金项目(21868007) （21868007）
广西创新驱动发展专项资金项目(桂科AA19254025) （桂科AA19254025）
中国科学院科技服务网络计划STS项目(KFJ-STS-QYZD-200 （）
KFJ-STS-QYZD-201) （）
广西自然科学基金项目(2021GXNSFBA196087 （）
2021AC19176) （）
广西重点研发计划项目(桂科AB19259001) （桂科AB19259001）
广西科学院基金项目(CQ-D-2415 （）
2021YFJ1210) （）
南宁市科学研究与技术开发计划项目(20206057) （20206057）
广西农业职业技术大学科研项目(XKJ2320) （XKJ2320）
