现代食品科技2025，Vol.41Issue(6)：298-308,11.DOI:10.13982/j.mfst.1673-9078.2025.6.0481
赶黄草总黄酮的安全性评价
Safety Evaluation of Total Flavonoids from Penthorum chinense Pursh
摘要
Abstract
The safety of total flavonoids of Penthorum chinense Pursh(TFPCP)was evaluated.An acute oral toxicity test,three genotoxicity tests,and a 28-day feeding study in rats were conducted to investigate the toxicological safety of TFPCP according to GB 15193-2014 Procedures for Toxicological Assessment of Food and Technical Guidelines for Toxicological Examination and Evaluation of Health Food and its Raw Materials(2020 Edition).These oral acute toxicity studies in mice revealed that the median lethal dose(LD50)of TFPCP was greater than 10 g/(kg·BW),suggesting that TFPCP is non-toxic.In the bacterial reverse mutation(Ames)test,all dose groups produced negative results.Additionally,for all dose groups,the colony counts did not exceed twice those of the solvent controls for TA97A,TA98,and TA100 or three times that for TA1535(P>0.05).Gavage administration of TFPCP in mice at doses of 2.4,1.2,and 0.6 g/(kg·BW)revealed no significant differences in mammalian erythrocyte micronucleus and chromosomal aberration test results in mouse spermatocytes compared with the negative control(P>0.05).Three different doses of TFPCP(2.4,1.2,and 0.6 g/(kg·BW))were administered to rats by gavage once daily for 28 consecutive days.No significant differences were observed between treated and control groups in terms of body weight,routine urinary and blood parameters,blood biochemical indices,and major organ coefficients(P>0.05).Similarly,histopathological examination of liver and kidney tissues revealed no significant pathological changes.Thus,TFPCP exhibited no acute toxicity,genotoxicity,or subacute toxicity under the conditions examined in this research and demonstrated excellent safety.These findings provide a scientific basis for development of TFPCP as a functional health food.关键词
赶黄草总黄酮/急性经口毒性/遗传毒性/28d经口毒性Key words
total flavonoids of Penthorum chinense Pursh/acute oral toxicity/genotoxicity/28-day oral toxicity引用本文复制引用
蒋屿,周希,康建英,刘洪盛,李舒婷,袁叶飞,付满玲..赶黄草总黄酮的安全性评价[J].现代食品科技,2025,41(6):298-308,11.基金项目
四川省重点研发计划项目(2022YFS0436) （2022YFS0436）
四川省自然科学基金项目(2022NSFSC1738) （2022NSFSC1738）
泸州市科技计划项目(2023SYF120 （）
2021-JYJ-109) （）
四川省中医药管理局中医药科研专项课题(2020CP0029) （2020CP0029）
西南医科大学-泸州市中医医院基地项目(2019-LH003) （2019-LH003）
西南医科大学产学研合作培育项目(2022CXY01) （2022CXY01）
西南医科大学大学生国家级创新创业训练计划项目(202310632074) （202310632074）
西南医科大学校级项目(2021KQN023) （2021KQN023）
精细化工应用技术泸州市重点实验室开放课题(HYJY-2106-B) （HYJY-2106-B）
