现代食品科技2025，Vol.41Issue(6)：309-317,9.DOI:10.13982/j.mfst.1673-9078.2025.6.0499
基于氨基化多壁碳纳米管-d-SPE-LC-MS/MS测定油籽油料中的10种真菌毒素
Determination of 10 Mycotoxins in Oilseeds by d-SPE-LC-MS/MS using Amine-functionalized Multi-walled Carbon Nanotubes
摘要
Abstract
Mycotoxin contamination in oilseeds is of particular concern because of their potential adverse effects,hence reliable analytical methods are required for detection and quantification.This study aims to develop a rapid method for the detection of ten mycotoxins in oilseeds using amine-functionalized multi-walled carbon nanotubes(NH2-MWCNTs)as adsorbents for dispersive solid-phase extraction(d-SPE)coupled with liquid chromatography-tandem mass spectrometry(LC-MS/MS).The structural and morphological characteristics of NH2-MWCNTs were analyzed using X-ray diffraction method,fourier transform infrared spectroscopy,and transmission electron microscopy,confirming increased surface defects after modification.The optimal pretreatment conditions were identified as 2%(V/V)formic acid in acetonitrile-water(80:20)as the extraction solvent and 20 mg NH2-MWCNTs as the adsorbent.The 10 mycotoxins exhibited good linearity within 1-20 μg/kg.The detection and quantitation limits ranged from 0.04 to 0.19 μg/kg and 0.13 to 0.63 μg/kg,respectively.The spiked recovery rates were 70.33%～111.67%and 0.55%～9.60%,respectively.The proposed method is simple,rapid,and environmentally friendly,substantially reducing pre-treatment time and organic solvent consumption.Utilization of NH2-MWCNTs could be an effective approach for determining various mycotoxins in oilseeds.关键词
真菌毒素/油籽油料/多壁碳纳米管/液相色谱串联质谱Key words
mycotoxins/oilseeds/multi-walled carbon nanotubes/LC-MS/MS引用本文复制引用
王思源,吴小勇,姚伟琴,舒圣元,巩志国,王静静..基于氨基化多壁碳纳米管-d-SPE-LC-MS/MS测定油籽油料中的10种真菌毒素[J].现代食品科技,2025,41(6):309-317,9.基金项目
新疆维吾尔自治区自然科学基金资助项目(2021D01A34) （2021D01A34）
