摘要
Abstract
To investigate the effects of different highland barley distiller's grains(HBDG)active extracts on mice with alcoholic liver injury(ALI),HBDG polypeptides and HBDG polyphenols were extracted from HBDG powder,and ALI C57BL/6 J mice model was es-tablished through continuous astringent administration of 52° Tianyoude liquor for 14 days.The time of disappearance and recovery of in-toxicated mice were observed and recorded;the morphopathological changes of liver were monitored by HE staining;serum and liver bio-chemical indices were measured by assay kit;and changes in liver metabolites were detected based on non-target metabolomics.Results showed that HBDG peptide and HBDG polyphenols intervention significantly reduced wake-up time(P＜0.05)and improved inflammato-ry cell infiltration and lipid vacuolization caused by prolonged drinking.Compared with the control group,serum aspartate aminotransferase(AST)and triglyceride(TG)were significantly reduced(30.83％,44.35％)(P＜0.05),while hepatic acetaldehyde dehydrogenase(ALDH)and superoxide dismutase(SOD)were remarkably increased(56.23％,49.96％)(P＜0.05)in the HBDG peptide group.At this time,AST and TG of HBDG polyphenol experimental group were remarkably decreased(29.08％,33.87％)(P＜0.05),and he-patic ALDH and SOD increased(12.80％,11.21％).In addition,ALDH and SOD levels were significantly improved(29.08％,33.87％)(P＜0.05)in the experimental group compared with the HBDG polyphenol.Subsequently,the capacity of HBDG peptides to degrade alcohol and act as antioxidants surpassed that of HBDG polyphenols,showing a notable disparity(P＜0.05).Analysis of liver metabolomics revealed that HBDG polypeptides could improve alcohol-induced liver damage by interfering with the arachidonic acid meta-bolic pathway to up-regulate the content of hepatic metabolites like prostaglandin F2α.Additionally,HBDG polyphenol might ameliorate alcohol-related liver by interfering with the linoleic acid metabolic to up-regulate 13-OxoODE and 8(R)-Hydroperoxylinoleic acid and im-pact thiamine metabolism to increase thiamine content,thereby alleviating alcohol-related liver harm.In summary,the use of HBDG pep-tides and HBDG polyphenols proved beneficial in enhancing ALI through the hastening of alcohol metabolism,bolstering antioxidant abili-ties,and controlling liver metabolic routes,with HBDG peptides outperforming HBDG polyphenols.The results can provide a theoretical basis for the study of new anti-alcohol and liver protection products.关键词
青稞酒糟/多肽/多酚/酒精性肝损伤/保肝作用Key words
highland barley distiller's grains/polypeptide/polyphenol/alcoholic liver injury/hepatoprotective effect
