食品与发酵工业2025，Vol.51Issue(13)：101-109,9.DOI:10.13995/j.cnki.11-1802/ts.040671
西藏牦牛酸奶中优良乳杆菌的分离筛选及益生性能研究
Isolation and screening of Lactobacillus from Xizang yak yogurt and its beneficial properties
摘要
Abstract
The aim of this study was to isolate and screen probiotic strains with potential immune functions from Xizang yak yogurt and to investigate their in vitro probiotic properties.The 12 strains of Lactobacillus isolated and screened were identified by morphological characterization and molecular biology,and the probiotic indicators such as acid tolerance,bile salt tolerance,simulated artificial gastro-intestinal fluid tolerance and antibiotic resistance were determined on the basis of adhesion and extracellular polysaccharide production,which were indirectly related to immune ability.The results showed that all 12 strains of Lactobacillus had good acid and bile salt toler-ance,and Lactobacillus pentosus S139-3 had the highest growth activity at pH 2.5 and 0.5％bile salt,with OD600 values of 0.34 and 0.51,respectively;the survival rate of L.pentosus S1 15-8 was the highest at 86.93％after simulated artificial gastric fluid treatment for 3 h,and the survival rate of S1 15-6 was the highest at 70.35％in intestinal fluid.The highest survival rate was 70.35％;all strains were highly resistant to levofloxacin,ciprofloxacin,streptomycin,and gentamicin;S139-3 had the highest self-aggregation and hydrophobicity of 34.46％and 41.59％,respectively,and may have high adhesion properties;and S1 15-6 yielded the highest content of extracellular poly-saccharides of 1.12 g/L.Therefore,the combined analysis of S1 15-6 and S139-3,two strains of Lactobacillus bacterium,had better probi-otic properties and could be used as excellent potential strains for the subsequent development of Lactobacillus with immune function and probiotic preparations.关键词
牦牛酸奶/乳杆菌/分离筛选/益生性/免疫Key words
yak yogurt/Lactobacillus/isolation and screening/probiotics/immunity引用本文复制引用
李菊梅,薛洁,于佳俊,文华英,贾福晨,黄本宸,唐雅利,王雅凝,张静,张玉红..西藏牦牛酸奶中优良乳杆菌的分离筛选及益生性能研究[J].食品与发酵工业,2025,51(13):101-109,9.基金项目
西藏自治区科技重大专项(XZ202201ZD0001N) （XZ202201ZD0001N）
