食品与发酵工业2025，Vol.51Issue(14)：167-174,8.DOI:10.13995/j.cnki.11-1802/ts.040876
赤松茸酸性多糖的结构表征及抗氧化活性研究
Structural characterization and antioxidant activity of acidic polysaccharides from Stropharia rugosoannulata
摘要
Abstract
This study aimed to investigate the in vitro antioxidant activity and structural characteristics of polysaccharides from Stro-pharia rugosoannulata.Through hot water extraction,separation,and purification,a homogeneous polysaccharide component(Stropharia rugosoannulata acidic polysaccharides,SRAP)was obtained from Stropharia rugosoannulata.The molecular weight,functional groups,glycosidic bonds,conformation,and branching of SRAP were characterized using high-performance liquid chromatography,gas chromatog-raphy-mass spectrometry,infrared spectroscopy,and other methods.The antioxidant activity in vitro of SRAP was evaluated by measuring its DPPH free radical,ABTS cationic radical,and hydroxyl radical scavenging abilities.Results indicated that SRAP was a heteropolysac-charide composed of mannose,glucuronic acid,glucose,and galactose,with a molecular weight of 2.57 × 104 Da and a total sugar con-tent of(88.47±0.74)％.SRAP did not have a triple helix structure but might contain more side chains and branches.The results of peri-odic acid oxidation and Smith degradation suggested that the connection modes of SRAP might contain 1→2,1→3,1→4 and 1→6 glyco-sidic bonds.The in vitro antioxidant results showed that SRAP exhibited strong DPPH free radical,ABTS cationic radical,and hydroxyl radi-cal scavenging abilities.The IC50 values of SRAP for DPPH free radicals,ABTS cationic radical,and hydroxyl radical were 0.34 mg/mL,0.79 mg/mL,and 1.18 mg/mL,respectively.The high antioxidant activity of the acidic polysaccharide from Stropharia rugosoannulata may be attributed to its lower molecular weight and higher proportion of galactose.This study could provide a theoretical basis for the fur-ther development and utilization of Stropharia rugosoannulata in Bo'ai county.关键词
赤松茸/酸性糖/结构表征/抗氧化Key words
Stropharia rugosoannulata/acidic polysaccharides/structural characterization/antioxidant引用本文复制引用
魏磊,李宁洁,金饶,谢晓阳,刘雨晴,王伟,董跟来,邱海亮,景炳年..赤松茸酸性多糖的结构表征及抗氧化活性研究[J].食品与发酵工业,2025,51(14):167-174,8.基金项目
河南省科技攻关项目(242102110135,242102310386) （242102110135,242102310386）
河南省科学院基本科研项目(230613074) （230613074）
