食品与发酵工业2025，Vol.51Issue(14)：195-201,7.DOI:10.13995/j.cnki.11-1802/ts.040442
环境胁迫对类植物乳植杆菌L-ZS9合成胞外多糖能力的影响
Effect of environmental stress on ability of Lactiplantibacillus paraplantarum L-ZS9 to synthesize exopolysaccharides
摘要
Abstract
Lactic acid bacteria exopolysaccharides have numerous probiotic functions,such as regulating intestinal flora,regulating immunity,and alleviating lactose intolerance.However,their production faces various environmental stresses in industrial applications and the human gastrointestinal system,including pH,bile salt,low temperature,etc.,resulting in low yield and economic benefits of exopo-lysaccharides.The purpose of this study was to explore the effects of acid,bile salt,low temperature,and ultra-high pressure stress on the ability of lactic acid bacteria to produce exopolysaccharides.Additionally,this study aimed to improve the production of exopolysaccharides by subjecting lactic acid bacteria to various environmental stresses and expanding their applications.Results showed that under acid stress,Lactiplantibacillus paraplantarum L-ZS9 increased its EPS production capacity with decreasing pH.The strain had the strongest EPS pro-duction capacity under pH 3.0 acid stress.The EPS production capacity of L-ZS9 was improved under bile salt stress,and the EPS pro-duction ability of a single colony was the strongest when the bile salt concentration was 1 g/L bile salt stress.However,both acid and bile salt could promote EPS production by L-ZS9 within certain limits.They also had detrimental effects when exceeding those limits for in-stance causing strain death under conditions of pH 2.0 or a bile salt concentration of 5 g/L.Under low-temperature stress,there was no significant improvement in the EPS production capacity of L-ZS9 observed.On the other hand,under ultra-high pressure stress,L-ZS9 demonstrated an increase in its EPS production capacity with increasing pressure,with a single colony showing its strongest EPS production at 300 MPa.In conclusion,environmental stress can be beneficial for improving the ability of L-ZS9 to produce exopolysaccharides.关键词
类植物乳植杆菌/胞外多糖/酸胁迫/胆盐胁迫/低温胁迫/超高压胁迫Key words
Lactiplantibacillus paraplantarum/exopolysaccharides/acid stress/bile salt stress/low temperature stress/ultra-high pres-sure stress引用本文复制引用
袁露,宋云龙,刘蕾,杨耀,张成毅,黎小丽,龙海祺,饶瑜..环境胁迫对类植物乳植杆菌L-ZS9合成胞外多糖能力的影响[J].食品与发酵工业,2025,51(14):195-201,7.基金项目
四川省自然科学基金项目(2023 NSFSC1203) （2023 NSFSC1203）
甘肃省科技重大专项(23ZDNA007) （23ZDNA007）
