食品与发酵工业2025，Vol.51Issue(14)：202-209,8.DOI:10.13995/j.cnki.11-1802/ts.040666
苹果渣低聚木糖的体外模拟胃肠消化-酵解特性
Characteristics of in vitro digestion and fermentation of xylooligosaccharide from apple pomace
摘要
Abstract
Natural oligosaccharides own a variety of active functions.Digestive and glycolysis properties of xylooligosaccharide(XOS)from apple pomace in vitro were explored to clarify its probiotic potential and to provide a new application direction for the comprehensive utilization of apple pomace.The changes in carbohydrate,pH,and OD values and short-chain fatty acid(SCFAs)contents were measured in the simulated gastrointestinal digestion and fermentation test.Microbial diversity analysis and 16 s RNA high-throughput sequencing were included to determine alterations of intestinal microbiota.Results showed that the contents of total sugar,reducing sugar and XOS did not significantly change in the gastrointestinal digestion stage,but significantly decreased after in vitro fermentation(P＜0.05),which in-dicated that the digestion of XOS mainly occurred in the fermentation stage.The pH value of fermentation broth in the XOS group de-creased significantly(P＜0.05).The contents of SCFAs,acetic acid,propionic acid,and n-butyric acid were extremely significantly higher than those in the other groups(P＜0.001).OD600 value was significantly higher than that in the CK group(P＜0.05).Results in-dicated that apple pomace XOS could promote the production of SCFAs and reduce the pH value,which could be beneficial to the growth of intestinal microorganisms.Microbial diversity analysis showed that XOS could improve the diversity and evenness of intestinal microbio-ta.The composition of intestinal microbiota in the XOS group was significantly different from the other two groups.At the phylum classifica-tion level,the relative abundance of firmicutes increased in the XOS group.At the genus classification level,the abundance of paracoides in the XOS group and the abundance of koala bacillus in the XOS group increased,separately compared with the CK group and with the PC group.The species abundance in the XOS group was lower than that in the CK group,which indicated that apple residue XOS could af-fect the composition of intestinal microbiota.Results of this study provide theoretical support for the application of XOS from apple pomace in food and medicine.关键词
苹果渣/低聚木糖/体外消化/酵解/肠道菌群Key words
apple pomace/xylo-oligosaccharide/in vitro digestion/glycolysis/intestinal flora引用本文复制引用
蔡露阳,戴沙,李艾莲,赵燕妮,陈雪峰..苹果渣低聚木糖的体外模拟胃肠消化-酵解特性[J].食品与发酵工业,2025,51(14):202-209,8.基金项目
陕西省科技厅重点研发一般项目(2022NY-014) （2022NY-014）
陕西省农业厅科技创新驱动项目(NYKJ-2022-XA-09) （NYKJ-2022-XA-09）
