食品与发酵工业2025，Vol.51Issue(14)：228-235,中插1,9.DOI:10.13995/j.cnki.11-1802/ts.042100
基于网络药理学及分子对接探讨白桦茸防治阿尔兹海默病的潜在作用机制
Potential mechanism of chaga mushroom in treatment of Alzheimer's disease based on network pharmacology and molecular docking
摘要
Abstract
To investigate the potential mechanisms of Chaga mushroom in the treatment of Alzheimer's disease(AD),network phar-macology and molecular docking techniques were used to predict the active components,key targets,and signalling pathways.The active components and target genes of Inonotus obliquus were obtained from databases such as TCMSP and literature.The disease targets of AD were acquired through databases such as GeneCards to identify the common targets of Inonotus obliquus and Alzheimer's disease.The pro-tein-protein interaction(PPI)network diagram of common targets was constructed using the String database,and the interaction network of"active components-key targets"was constructed by Cytoscape software.GO and KEGG enrichment analyses were performed by DAVID and Metascape databases.Molecular docking simulations were conducted using Autodock software.Finally,9 active components and 3 397 targets of Alzheimer's disease were screened out.Twenty key targets were obtained by PPI,such as MMP3,IL6,AKT1,ILI0,TNF,MAPK1,HSP90AB1,and so on.KEGG pathway analysis showed that Inonotus obliquus played an anti-AD role through the signalling pathways of lipid and atherosclerosis,hepatitis B,human cytomegalovirus infection,interleukin-17,Alzheimer's disease,and so on.GO enrichment analysis showed that it was related to RNA polymerase Ⅱ transcription factor complex,an integral component of the plasma membrane,positive regulation of pri-miRNA transcription from RNA polymerase Ⅱ promoter,and so on.20 key targets were docked with their corresponding active components to verify that they could bind stably,which predicted that quercetin played an important role in allevi-ating Alzheimer's disease.Chaga mushroom could interfere with multiple signalling pathways,such as lipid and atherosclerosis,as well as through multiple components and targets,providing theoretical support for the development of medicine and food homologous health foods.关键词
桦褐孔菌/药食同源/老年痴呆/蛋白质-蛋白互作/信号通路Key words
Inonotus obliquus/homology of medicine and food/senile dementia/protein-protein interaction/signalling pathway引用本文复制引用
符丹丹,邵鑫源,张琦,张迪,彭心暖,刘燕山,孙建瑞,宫强..基于网络药理学及分子对接探讨白桦茸防治阿尔兹海默病的潜在作用机制[J].食品与发酵工业,2025,51(14):228-235,中插1,9.基金项目
河南省自然科学基金项目(242300420145) （242300420145）
河南省科技攻关项目(232102110134) （232102110134）
