中国食物与营养2025，Vol.31Issue(6)：58-64,7.
石斛内生菌产石斛碱类生物碱发酵培养条件优化及降血糖活性研究
Optimization of Fermentation Culture Conditions and Hypoglycemic Activity of Endophytic Actinomycetes Producing Dendrobium Alkaloids
摘要
Abstract
[Objective]To optimize the fermentation culture conditions of endophyte Pseudomonas protegens CM-YJ 44 to improve the production of the dendrobium alkaloids.[Method]The single factor and orthogonal experiment were used to optimize the fermentation culture conditions,and HPLC was used to select the optimal culture conditions with the highest production.Insulin Resistant human hepatocellular carcinoma model induced by palmitic acid(PA)combined with hyper insulin were used investigate the hypoglycemic bioactivity of secondary metabolites of this strain.With the Metformin(Met)as a positive control and the intake of 2-(n-7-nitro-2,1,3-benzooxadiazol-4-amino)-2-deoxy-d-glucose(2-NBDG)was used as a reference point.[Result]The optimal fermentation medium was as follows,yeast powder 3 g/L,malt powder 5 g/L,fructose 5 g/L,NaCl 1 g/L,vitamin B1 0.5 g/L,initial pH value 6.5.And the highest yield of dendrobium alkaloids from strain CM-YJ 44 reached at 39.86 mg/mL when cultured with 28℃for 48 h at the speed of 200 rpm/min.It was also found that under the treatment of 50 and 100 ng/mL dendrobium and secondary metabolites,the cell viability were 101%and 91.62%,respectively,and the uptake of 2-NBDG was significantly increased.[Conclusion]The yield of dendrobium alkaloids was the highest under the optimum culture conditions.The hypoglycemic activity of the secondary metabolites of the strain was better than that of Dendrobium at the concentration of 50 and 100 ng/mL,and both of them were better than Met.关键词
石斛内生菌/工艺优化/石斛碱类生物碱/HepG2/降血糖Key words
endophyte of dendrobium/process optimization/dendrobium alkaloids/human hepatocellular carcinoma(HepG2)cells/hypoglycemic引用本文复制引用
鲁为,刘佳萌,覃璐琪,郑佳欢,郭润芳,范蓓..石斛内生菌产石斛碱类生物碱发酵培养条件优化及降血糖活性研究[J].中国食物与营养,2025,31(6):58-64,7.基金项目
国家重点研发计划(项目编号:2022YFD1600303) （项目编号:2022YFD1600303）
中国农业科学院农产品加工研究所创新工程院所重点任务(项目编号:CAAS-ASTIP-G2022-IFST-06). （项目编号:CAAS-ASTIP-G2022-IFST-06）
