中外葡萄与葡萄酒Issue(4)：6-13,8.DOI:10.13414/j.cnki.zwpp.2025.04.002
葡萄Vv4CL基因家族鉴定及组织特异性表达分析
Identification and Tissue-specific Expression Analysis of Vv4CL Gene Family in Grapevine
摘要
Abstract
4-Coumarate-CoA ligase(4CL)is a pivotal enzyme in the plant phenylpropanoid metabolic pathway,playing a critical role in the biosynthesis of flavonoids and lignin,which significantly influence grape berry quality and stress resistance.Through bioinformatics analysis,15 members of the 4CL gene family(Vv4CL01-Vv4CL15)were identified in grapevine,primarily distributed across seven chromosomes,including Chr13 and Chr18.Phylogenetic analysis classified these genes into four subfamilies(I-IV).Cis-acting element analysis revealed an enrichment of regulatory elements related to growth and development,hormone response,and stress resistance in the promoter regions of 4CL genes.Collinearity analysis indicated that segmental duplication was the major driver of family expansion.qRT-PCR results demonstrated Vv4CL02 and Vv4CL06 were highly expressed in the rachis of Kyoho grape,while Vv4CL03 exhibited the highest expression levels in young berries and stems of Shine Muscat grape.This study provides a foundation for further exploration of the mechanisms by which the 4CL gene family participates in grapevine responses to various abiotic stresses.关键词
葡萄/Vv4CL基因家族/组织特异性/表达分析Key words
grapevine/Vv4CL gene family/tissue specificity/expressionanalysis分类
农业科技引用本文复制引用
王超霞,胡玉龙,马欣荣,田淑芬,王荣,马闯,姜建福..葡萄Vv4CL基因家族鉴定及组织特异性表达分析[J].中外葡萄与葡萄酒,2025,(4):6-13,8.基金项目
财政部和农业农村部:国家现代农业产业技术体系(CARS-29-zp-1) （CARS-29-zp-1）
天津市教委科研计划项目(2022KJ025) （2022KJ025）
