粮油食品科技2025，Vol.33Issue(4)：39-48,10.DOI:10.16210/j.cnki.1007-7561.2025.04.007
超声辅助酸热处理制备豌豆蛋白纤维及其结构表征
Ultrasonic-Assisted Acid Heat Treatment for the Preparation and Structural Characteristics of Pea Protein Fibers
摘要
Abstract
In this study,pea protein isolate(PPI)was used as the raw material to prepare pea protein isolate fibrils(PPIF)through ultrasound-assisted acid-thermal treatment.The effects of ultrasound and acid-thermal treatment on the physicochemical properties and structural characteristics of PPIF were systematically investigated.The results revealed that after ultrasonic pretreatment,the average particle size of PPI decreased by 32 nm,and the thioflavin T fluorescence intensity increased during fibrillation,indicating that ultrasound pretreatment could reduce the activation energy required for fibril formation,thereby facilitating protein fibrillation.After ultrasonic treatment,the β-sheet content of PPIF increased from 42.67±1.76%to 44.70±0.79%,suggesting that ultrasound pretreatment enhanced hydrogen bonding and hydrophobic interactions,inducing secondary structural transitions in PPIF and promoting the formation and ordered assembly of PPIF aggregates.Acid-thermal treatment accelerated protein hydrolysis and subunit degradation,further promoting the formation of PPIF.The ultrasound-assisted acid-thermal treatment increased the solubility of PPI from 36.5%to 70.1%,while the emulsion stability index(ESI)and emulsifying activity index(EAI)increased by4.69 m2/g to 6.31 m2/g and 39.44 min to 59.33 min,respectively,significantly enhancing the solubility and emulsifying capacity of PPI.These findings suggest that ultrasound pretreatment enhances the self-assembly capability of PPIF,potentially broadening the application of PPI in novel functional foods.关键词
豌豆蛋白/蛋白纤维/超声处理/酸热处理/理化性质Key words
pea protein/protein fibrils/sonication treatment/acid-heat treatment/physicochemical properties分类
轻工纺织引用本文复制引用
任玉凯,牟松松,李志,白珂菲,刘欣怡,王雨生,陈海华..超声辅助酸热处理制备豌豆蛋白纤维及其结构表征[J].粮油食品科技,2025,33(4):39-48,10.基金项目
山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(2023TZXD078) （乡村振兴科技创新提振行动计划）
日照市创新领军人才(团队)(日政字[2021]64号) （团队）
青岛农业大学研究生创新项目(QNYCX23082) （QNYCX23082）
青岛农业大学大学生创新创业训练计划项目(QNDC20240019) The Provincial Key Research and Development(R&D)Plan Projects of Shandong Province-Technological Innovation Plan Project(No.2023TZXD078) （QNDC20240019）
Project of Rizhao Innovation Leading Talent(Team)(No.Rizheng[2021]-64) （Team）
Qingdao Agricultural University Postgraduate Creative Education Program(No.QNYCX23082) （No.QNYCX23082）
The Innovation and Entrepreneurship Training Program for College Students of Qingdao Agricultural Univeristy(No.QNDC20240019) （No.QNDC20240019）
