食品科学技术学报2025，Vol.43Issue(4)：61-76,114,17.DOI:10.12301/spxb202500043
1株植物乳植杆菌对酒精性肝损伤的作用研究
Effect of a Lactiplantibacillus plantarum Strain on Alcoholic Liver Injury
摘要
Abstract
Lactiplantibacillus plantarum is a probiotic with multiple health benefits.To investigate its effects against alcoholic liver injury,16 strains of Lactiplantibacillus plantarum were isolated from traditional fermented foods.Their ethanol degradation capacity and effects on alcohol dehydrogenase and acetaldehyde dehydrogenase activities were evaluated,and 8 strains that demonstrated high alcohol metabolism rates and reduced hepatic oxidative stress levels were preliminarily screened.Subsequent assessment of antioxidant capacity,acid resistance,and bile salt tolerance of these 8 strains was carried out,and an optimal strain,Lactiplantibacillus plantarum FS5-5 was identified.An alcoholic liver injury mouse model was established to investigate the effects of Lactiplantibacillus plantarum FS5-5.Results showed that FS5-5 exhibited a strong alcohol metabolism capability with alcohol dehydrogenase and acetaldehyde dehydrogenase activities reaching 35.99 U/mL and 162.11 U/mL,respectively.Meanwhile,high-dose FS5-5 significantly prolonged drunkenness duration,shortened sobering time,and reduced serum ethanol concentration(P＜0.05).Furthermore,the high-dose FS5-5 group(1× 109CFU/mL)notably alleviated pathological liver damage,decreased hepatic malondialdehyde and triglyceride levels,increased glutathione content,reduced serum transaminases and inflammatory factor levels,enhanced antioxidant enzyme activity,effectively mitigated hepatic oxidative stress(superoxide dismutase level)and inflammatory injury(IL-6,IL-1 β,TNF-α levels),and lowered total cholesterol content.Mechanistic studies demonstrated that this strain exerted hepatoprotective effects against alcoholic liver injury by regulating hepatic gene Nrf2,Cyp2e1,NF-κB,and Tlr4 expression,reducing pro-inflammatory cytokine levels,and alleviating oxidative stress and lipid accumulation.This research provided theoretical basis for developing functional foods with auxiliary protective effects against alcoholic liver injury.关键词
益生菌/酒精性肝损伤/氧化应激/炎症因子/谷胱甘肽Key words
probiotic/alcoholic liver injury/oxidative stress/inflammatory factors/glutathiones分类
轻工纺织引用本文复制引用
夏苏宁,乌日娜,李彤,杨海鑫,安飞宇,薛瑞霞,武俊瑞,迮晓雷..1株植物乳植杆菌对酒精性肝损伤的作用研究[J].食品科学技术学报,2025,43(4):61-76,114,17.基金项目
国家自然科学基金面上项目(32172279) （32172279）
辽宁省科学技术计划项目(2024JH2/101900005) （2024JH2/101900005）
沈阳农业大学引进人才(高层次)科研启动项目(2023YJRC002) （高层次）
沈阳市科技创新平台项目(21-103-0-14、21-104-0-28).National Natural Science Foundation of China(32172279) （21-103-0-14、21-104-0-28）
Liaoning Provincial Science and Technology Program Project(2024JH2/101900005) （2024JH2/101900005）
Shenyang Agricultural University High-Level Talent Introduction Scientific Research Startup Fund Project(2023YJRC002) （2023YJRC002）
Shenyang Science and Technology Innovation Platform Project(21-103-0-14,21-104-0-28). （21-103-0-14,21-104-0-28）
