食品科学技术学报2025，Vol.43Issue(4)：105-114,10.DOI:10.12301/spxb202400152
皮蛋模拟胃肠道消化物对HepG2细胞迁移和侵袭的影响机制
Mechanism of Preserved Eggs Simulated Gastrointestinal Digests on Migration and Invasion of HepG2 Cells
摘要
Abstract
The migration and invasion of tumor cells are important processes for the spread of tumors.In order to study the mechanism of consuming preserved eggs on the migration and invasion of human HepG2 cells,the effects of preserved eggs simulated gastrointestinal digests(PESD)on the migration and invasion of hepatoma HepG2 cells were studied by cell scratch test and Transwell cell invasion test.Western blot method was used to determine the expression of cell migration and invasion related factors in HepG2 cells induced by PESD.The process of epithelial-mesenchymal transition(EMT)induced by PESD was studied by real-time fluorescence quantitative PCR.The results showed that PESD inhibited the migration of HepG2 cells in a time-dose dependent manner.At 72 h,the scratch healing rate of control group was 3 times higher than that of 4 mg/mL PESD group.The invasion of HepG2 cells was inhibited in a dose-dependent manner.When PESD was 4 mg/mL,the number of HepG2 cells passing through the membrane was only 20.33±3.06,which was significantly higher than that of the control group(P＜0.001).Further study found that the inhibitory mechanism was that PESD affected the Wnt/β-catenin signal pathway by reducing the expression of β-catenin protein.By increasing the expression of E-cadherin gene in HepG2 cells,reducing the expression of N-cadherin and Vimentin genes,inhibiting the process of EMT in HepG2 cells,increasing intercellular adhesion and the expression of matrix metalloproteinase and 9,the degradation of extracellular matrix and basement membrane was reduced,thus the migration and invasion of HepG2 cells was inhibitted.The research aimed to provide theoretical basis for exploring the health effects of preserved eggs.关键词
皮蛋/HepG2细胞/细胞迁移/细胞侵袭/上皮-间充质转化过程Key words
preserved egg/HepG2 cell/cell migration/cell invasion/epithelial-mesenchymal transition process分类
轻工纺织引用本文复制引用
张研,朱进,朱悦,王晶,赵怡欣,何立超,靳国锋..皮蛋模拟胃肠道消化物对HepG2细胞迁移和侵袭的影响机制[J].食品科学技术学报,2025,43(4):105-114,10.基金项目
国家自然科学基金资助项目(32472369).National Natural Science Foundation of China(32472369). （32472369）
