食品与发酵工业2025，Vol.51Issue(15)：9-14,6.DOI:10.13995/j.cnki.11-1802/ts.042593
毕赤酵母Ku70基因缺失对(-)-α-红没药醇合成的影响
Effect of Ku70 deletion on the(-)-α-bisabolol synthesis in Pichia pastoris
摘要
Abstract
(-)-α-bisabolol(BO)is widely used in the pharmaceutical and cosmetics industries due to the anti-inflammatory,anti-bacterial,and skin health improving effects.So,the microbial synthesis of BO has important prospects.At present,BO synthesis has been reported in Escherichia coli,Bacillus subtilis,Yarrowia lipolytica,and Saccharomyces cerevisiae.These metabolic engineering modification strategies include strengthening the mevalonate pathway and β-oxidation pathway,increasing acetyl CoA pool,weakening competitive path-ways and so on.Based on this,Pichia pastoris was employed for the first time to produce BO by through gene metabolic engineering and evaluated the effect of knocking out Ku70(DNA repair encoding non homologous end connections)strategy on BO.Firstly,MrBBs(enco-ding BO synthase)was transformed into GS115 to construct GS115/pPICZ A-MrBBs.Meantime,two phase culture was carried out with the addition of n-dodecane.After 132 h,the BO synthesis reached 3.11 mg/L.Then,GS115ΔKu70 was obtained using pPpT4 plasmid,and GS115ΔKu70/pPICZ A-MrBBs was constructed.After 132 h,the BO was synthesized at 83.22 mg/L,increasing by 15.95 times com-pared with GS115/pPICZ A-MrBBs,which indicated that the deletion of Ku70 gene can significantly improve BO synthesis.This study shows that P.pastoris has the potential for BO synthesis,and knocking out Ku70 can significantly enhance BO synthesis,which lays a sol-id foundation for further metabolic engineering modification.关键词
毕赤酵母/(-)-α-红没药醇/敲除/Ku70/MrBBsKey words
Pichia pastoris/(-)-α-bisabolol/knockout/Ku70/MrBBs引用本文复制引用
蒋雨红,邱世骥,胡蝶,周培鹏,廖清,刘文宗,胡晓清..毕赤酵母Ku70基因缺失对(-)-α-红没药醇合成的影响[J].食品与发酵工业,2025,51(15):9-14,6.基金项目
国家重点研发计划政府间国际科技创新合作项目(2023YFE0104400) （2023YFE0104400）
