食品与发酵工业2025，Vol.51Issue(15)：15-23,中插1-中插2,11.DOI:10.13995/j.cnki.11-1802/ts.042443
解淀粉芽孢杆菌LS1-002-014对亚精胺的高效降解机制及环境适应性研究
Study on the high-efficiency degradation mechanism of spermidine and environmental adaptability of Bacillus amyloliquefaciens LS1-002-014
摘要
Abstract
Excessive accumulation of biogenic amines in fermented foods may pose health risks,and microbial degradation is an ef-fective strategy to reduce their accumulation.In this study,Bacillus amyloliquefaciens LS1-002-014 was taken as the research object.Through the integration of growth characteristic analysis,high-performance liquid chromatography,and whole-genome sequencing technol-ogy systems,the biogenic amine degradation characteristics and molecular mechanisms of this strain were systematically analyzed.The re-sults showed that the degradation efficiency of this strain for spermidine was significantly higher than that of other biogenic amines(P＜0.01),and the optimal degradation rate(35.91±2.09)％was achieved at 37℃.In the environmental stress experiment,the strain still maintained stable biogenic amine degradation activity in the range of 2.5％-7.5％NaCl mass fraction.However,the addition of nitrite(50-300 mg/L)had little effect on the degradation rate of spermidine,which was stable at(22.26±1.71)％.Whole-genome analysis showed that this strain was composed of a circular chromosome of 4 214 996 bp(with a GC content of 46.12％)and a plasmid of 7 410 bp,and carried key spermine metabolism gene clusters,such as speA(arginine decarboxylase),speB(agmatinase),speG(sper-midine N-acetyltransferase),and speE(spermidine synthase).In conclusion,strain LS1-002-014 has high-efficiency spermidine degrada-tion ability and strong environmental adaptability.This study provides a theoretical basis and candidate functional strains for the precise control of biogenic amines in fermented foods.关键词
解淀粉芽孢杆菌/生物胺降解率/全基因组分析/亚精胺/环境适应性Key words
Bacillus amyloliquefaciens/biogenic amine degradation rate/genome-wide analysis/spermidine/environmental adaptation引用本文复制引用
谢丽丽,王修俊,王雪郦,徐智虎,刘雪婷..解淀粉芽孢杆菌LS1-002-014对亚精胺的高效降解机制及环境适应性研究[J].食品与发酵工业,2025,51(15):15-23,中插1-中插2,11.基金项目
贵州省科技支撑项目(黔科合支撑[2021]一般273) （黔科合支撑[2021]一般273）
贵州省科技支撑项目(黔科合支撑[2021]一般121) （黔科合支撑[2021]一般121）
贵州省科技支撑项目(黔科合支撑[2022]一般148) （黔科合支撑[2022]一般148）
