食品与发酵工业2025，Vol.51Issue(15)：32-40,9.DOI:10.13995/j.cnki.11-1802/ts.042316
基于低聚半乳糖和两歧双歧杆菌的合生制剂改善脂多糖诱导的肠道屏障损伤
Improving effect of synbiotic preparation based on galactooligosaccharides and Bifidobacterium bifidum on lipopolysaccharide-induced intestinal barrier damage
摘要
Abstract
The modulatory effects of synbiotic agents composed of galactooligosaccharides(GOS)and Bifidobacterium bifidum on in-testinal barrier function remain unclear.This study aimed to establish a mouse model of intestinal barrier injury using lipopolysaccharide(LPS)and to investigate the synergistic effects and underlying mechanisms of these synbiotic agents in enhancing intestinal barrier func-tion.Mice were randomly assigned to the normal control,model,and GOS+B.bifidum CICC® 10395 groups.Key indicators,including intestinal permeability,tight junction protein expression,cytokine levels,mucin expression,and short-chain fatty acid content,were as-sessed.The regulatory role of short-chain fatty acids in barrier function was further explored at the cellular level.Compared to the model group,mice treated with GOS+B.bifidum CICC® 10395 exhibited significant reductions in serum FITC-dextran(45.24％),D-lactic acid(30.66％),and diamine oxidase(DAO,32.38％)concentrations.ZO-1 and ZO-2 mRNA expression levels were upregulated by 2.19-fold and 2.67-fold,respectively,whereas TNF-α,IL-1β,IL-6,and myeloperoxidase(MPO)activities decreased by 49.07％,54.18％,38.36％,and 34.69％.Moreover,MUC2,MUC1,MUC4,and MUC17 mRNA expressions increased by 2.55-fold to 9.10-fold.Furthermore,GOS+B.bifidum CICC® 10395 administration promoted the colonization of Bifidobacterium in the intestines and in-creased acetic acid production.Cellular experiments demonstrated that acetic acid enhanced the barrier function of Caco-2 monolayers and inhibited pro-inflammatory cytokine secretion in RAW264.7 macrophages.In conclusion,synbiotic preparations combining GOS and B.bifidum CICC® 10395 demonstrated efficacy in enhancing intestinal barrier integrity and reducing permeability,likely mediated through acetic acid-dependent mechanisms.关键词
低聚半乳糖/两歧双歧杆菌/肠道屏障/乙酸/细胞模型Key words
galactooligosaccharides/Bifidobacterium bifidum/intestinal barrier/acetic acid/cell model引用本文复制引用
单艺,王旻,董世荣,卞春..基于低聚半乳糖和两歧双歧杆菌的合生制剂改善脂多糖诱导的肠道屏障损伤[J].食品与发酵工业,2025,51(15):32-40,9.基金项目
黑龙江省重点研发计划指导类项目(GZ2024026) （GZ2024026）
哈尔滨学院青年博士科研启动基金项目(HUDF2021113) （HUDF2021113）
