食品与发酵工业2025，Vol.51Issue(15)：41-50,10.DOI:10.13995/j.cnki.11-1802/ts.042337
植物乳植杆菌GXFF202402调节小鼠肠易激综合征干预痔疮的效果
Effect of Lactiplantibacillus plantarum GXFF202402 on the intervention of hemorrhoids in mice with irritable bowel syndrome
摘要
Abstract
This study investigated the therapeutic potential of Lactiplantibacillus plantarum GXFF202402(LP-GXFF202402)in alle-viating hemorrhoid-associated colorectal damage through gut microbiota modulation in a mouse model.In this study,croton oil was used to establish a mouse experimental model,and cytokine levels in serum and rectal tissues of mice were detected.Pathological observation of colon and rectal tissues of mice was carried out by hematoxylin-eosin(HE)staining.The relevant expression of microorganisms in tissue and intestinal contents was also detected by quantitative real-time polymerase chain reaction(qRT-PCR)and western blot.The experimen-tal results showed that LP-GXFF202402 could reduce the anorectal swelling rate of hemorrhoid mice.Improve serum IL-2 level of hemor-rhoid mice and decrease serum levels of IL-4,IL-6,IL-1β,TNF-α and corticosterone(CORT).At the same time,it can also reduce the levels of IL-1β,IL-6,TNF-α,protease activated receptor 2(PAR-2),mast cell tryptase(MCT)and increase the levels of IL-10,Oc-cludin in rectal tissue of hemorrhoid mice.LP-GXFF202402 was also able to reduce the degree of colon and rectal lesions caused by hem-orrhoids in mice.Further experimental results also showed that LP-GXFF202402 can up-regulate Occludin and down-regulate PAR-2 mR-NA expression in colon tissue of hemorrhoid mice,and down-regulate IL-6 and TNF-α mRNA and protein expression in rectal tissue.The mRNA detection results of intestinal microorganisms showed that LP-GXFF202402 could increase the abundance of Firmicutes,Bifidobac-teria and Lactobacillus and decrease the intensity of Bacteroidetes in the intestinal tract of hemorrhoid mice.It can be seen that LP-GXFF202402 can effectively intervene in the colorectal damage caused by hemorrhoids,regulate intestinal microbes,and thus relieve hemorrhoids,and the effect is close to the hemorrhoid drug Zhishuning tablet.关键词
植物乳植杆菌/肠易激综合征/痔疮/IL-6Key words
Lactiplantibacillus plantarum/irritable bowel syndrome/hemorrhoids/interleukin-6(IL-6)引用本文复制引用
赵欣,谭晓红,刘新红,易若琨,龙兴瑶..植物乳植杆菌GXFF202402调节小鼠肠易激综合征干预痔疮的效果[J].食品与发酵工业,2025,51(15):41-50,10.基金项目
重庆市教委科学技术研究项目(KJQN202201614) （KJQN202201614）
重庆市留学人员回国创业创新支持计划项目(cx2022030) （cx2022030）
