摘要
Abstract
Acute kidney injury(AKI)is a common clinical renal disease,with inflammation identified a key factor contributing to kidney damage.This study established a cisplatin-induced mouse model of AKI and evaluated the effects of live bacteria,fermented super-natant,and a bacterial lysate of Limosilactobacillus reuteri DYNDL56M17(a strain with anti-inflammatory properties)on alleviating AKI in mice.Evaluation included examining renal histopathological sections and measuring the levels of-serum creatinine,blood urea nitrogen(BUN),antioxidants,and inflammatory cytokines.Compared to the model group,the fermented supernatant and bacterial lysate of L.reu-teri DYNDL56M17 significantly reduced creatinine and BUN levels.Specifically,the fermented supernatant reduced creatinine and BUN levels by 27.9％and 16.7％,respectively,whereas the bacterial lysate reduced these levels by 21.1％and 10.1％,respectively.Addi-tionally,the fermented supernatant effectively reduced oxidative damage in the kidneys,lowering the renal malondialdehyde content by 13.49％and decreasing the levels of inflammatory cytokines.These findings suggest that the fermented supernatant and bacterial lysate of L.reuteri DYNDL56M17 may alleviate AKI by upregulating the G-protein-coupled receptor GPR43 expression,thereby inhibiting inflam-mation and oxidative stress.关键词
急性肾损伤/菌体裂解物/发酵上清液/罗伊氏粘液乳杆菌/炎症Key words
acute kidney injury/probiotic lysates/fermentation supernatant/Limosilactobacillus reuteri/inflammation
