食品与发酵工业2025，Vol.51Issue(15)：66-73,8.DOI:10.13995/j.cnki.11-1802/ts.040851
脂肪酶抑制活性生姜内生菌的定性定量筛选及鉴定
Qualitative and quantitative screening and identification of endophytic ginger bacteria with lipase inhibitory activity
摘要
Abstract
Ginger has the effect of inhibiting lipid absorption,lowering blood pressure and preventing atherosclerosis.Endophytic bac-teria with lipase inhibitory activity were isolated and purified from ginger slices.Totally six active endophytic bacteria were qualitatively de-tected by TLC-bioautography,and five strains belonged to Pseudomonas,one belonged to Acinetobacter by 16S rRNA gene sequence com-parison.The p-nitrophenyl palmitate method was used to quantitatively analyze the inhibition rate of the 20 mL ethyl acetate extract of six strains of active bacteria on lipase.The incubation time was 5 min,the enzymatic reaction time was 15 min,and the reactants were added in the order of extraction,p-nitrophenyl palmitate,and enzyme.It was found that the inhibition rate of four strains was above 20％,among which SJ14 and SJ12 had higher inhibition rate,reaching(67.79±1.75)％and(48.28±0.73)％,respectively.The active compounds in the extract were detected by HPLC fingerprint.The aim of this study was to establish a new idea of qualitative and quantitative screening of microbial lipase inhibitors,and to provide scientific basis for obtaining potential production strains of highly effective lipid-lowering ac-tive substances and highly effective and low-toxic lipase inhibitors.关键词
生姜/内生菌/脂肪酶抑制活性/定性筛选/定量筛选Key words
ginger/endophytic bacteria/lipase inhibitory activity/qualitative screening/quantitative screening引用本文复制引用
彭涵,谢欣然,陈思维,李思轩,张家慧,任虹..脂肪酶抑制活性生姜内生菌的定性定量筛选及鉴定[J].食品与发酵工业,2025,51(15):66-73,8.基金项目
国家自然科学基金项目(31771960) （31771960）
