轻工学报2025，Vol.40Issue(4)：30-40,11.DOI:10.12187/2025.04.004
不同大麦多酚的抗氧化及降脂能力差异研究
Study on the differences in antioxidant and lipid-lowering activities of different barley polyphenols
摘要
Abstract
Barley polyphenols were prepared by fermentation(using Lactiplantibacillus plantarum dy-1)and enzymatic hydrolysis(with sulfatase),respectively.The phenolic profiles of fermented barley polyphenols(FBP)and enzymatic hydrolysis barley polyphenols(EBP)were analyzed.The antioxidant capacities and lipid-lowering effects of FBP and EBP were further investigated.The results showed that the total phenolic contents of EBP and FBP were 78.35 μg GAE/mL and 146.56 μg GAE/mL,respectively.The absolute concentrations of vanillin,benzoic acid,2,4-dihydroxybenzoic acid,epicatechin,and salicylic acid differed significantly between the two barley polyphenol preparations.Under equivalent total phenol content conditions,vanillin,chlorogenic acid,and gallic acid were characteristic components of EBP,whereas benzoic acid,3,4-dimethoxybenzoic acid,and epicatechin were characteristic components of FBP.Compared to EBP,the DPPH and ABTS+scavenging capacities of FBP were enhanced by 36.8%and 13.3%,respectively.At a total phenolic content of 20 μg GAE/mL,both FBP and EBP effectively reduced intravital lipid droplet,accumulation and decreased triglyceride(TG,23.3%and 27.9%reduction,respectively)levels in Caenorhabditis elegans.Furthermore,both FBP and EBP enhanced locomotor activity in C.elegans,thereby accelerating lipid catabolism,and exerted lipid-lowering effects by suppressing the aberrant activation of the SBP-1 signaling pathway.Notably,EBP showed a more pronounced inhibitory effect on the insulin/insulin-like growth factor signaling pathway compared to FBP(P<0.05).In conclusion,under equivalent total phenolic content conditions,the compositional differences of barley polyphenols significantly modulated their antioxidant capacities and lipid-lowering activities.关键词
微生物发酵/酶法水解/大麦多酚/抗氧化能力/降脂能力/秀丽隐杆线虫Key words
microbial fermentation/enzymatic hydrolysis/barley polyphenols/antioxidant capacity/lipid lowering ability/Caenorhabditis elegans分类
轻工纺织引用本文复制引用
雷月馨,程张晨,陈瑾,贺禹丰,肖香..不同大麦多酚的抗氧化及降脂能力差异研究[J].轻工学报,2025,40(4):30-40,11.基金项目
江苏省重点研发计划项目(BE2022053) （BE2022053）
国家自然科学基金青年科学基金项目(32302047) （32302047）
中国博士后科学基金面上项目(2023M731378) （2023M731378）
镇江市创新能力建设计划——学科类重点实验室建设项目(SS2024005) （SS2024005）
