轻工学报2025，Vol.40Issue(4)：77-85,9.DOI:10.12187/2025.04.009
产香酵母的分离、鉴定及其在低次烟叶发酵中的应用
Isolation and identification of an aroma-producing yeast strain and its application in fermentation of low-grade tobacco leaves
摘要
Abstract
Aroma-producing microorganisms were isolated from high-temperature Daqu,followed by morphological observation,molecular biological identification,and growth profile analysis.A highly aroma-producing strain JQ-3 was identified as Cyberlindnera jadinii.The optimal growth conditions were 30℃,initial pH value 5,and shaking speed 180 r/min,under which the maximum cell density was achieved.After 24 h fermentation of low-grade tobacco leaves,the number of aroma components increased from 26 to 43,primarily alcohols,ketones,and esters,with concentrations 12.75-fold,2.25-fold,and 4.29-fold higher than those in the control group.Key aroma components,including phenyl ethanol,solanone,megastigmatrienone,and dihydroactinidiolide,showed significant increases.Compared to the control group,cigarettes supplemented with extracts from fermented low-grade tobacco leaves exhibited improved aroma quality,increased aroma intensity,a fuller aroma profile,enhanced coordination,and elevated sensory quality.关键词
产香酵母/鉴定/低次烟叶/香气成分/发酵Key words
aroma-producing yeast/identification/low-grade tobacco leaves/aroma component/fermentation分类
轻工纺织引用本文复制引用
宋丽丽,苏飘,章钰璟,魏涛..产香酵母的分离、鉴定及其在低次烟叶发酵中的应用[J].轻工学报,2025,40(4):77-85,9.基金项目
河南省重点研发专项项目(241111110400) （241111110400）
河南省科技攻关项目(242102110118) （242102110118）
中国烟草总公司科技项目(110202202026) （110202202026）
